CAJ | 학술논문

目的：研究疏肝健脾方（ SGJP）对CCl4诱发的化学肝损伤小鼠的保护作用。方法将小鼠随机分为6组：正常对照组、模型组、疏肝健脾方低剂量组、疏肝健脾方中剂量组、疏肝健脾方高剂量组、阳性药（联苯双酯， BP）组，给药组灌胃给药，每天1次，连续灌胃10 d，正常组和模型组用等量的溶媒，于末次给药8 h后，除正常组外其余每组腹腔注射0．1％CCl4橄榄油溶液（0．1 mL/10 g）复制小鼠化学性肝损伤模型，检测小鼠血清谷氨酸转氨酶（ALT）、天冬氨酸转氨酶（AST）、肝脏指数、肝组织超氧化物歧化酶（SOD）、丙二醛（MDA）、谷胱甘肽（GSH）含量，HE染色观察肝组织形态学变化。结果疏肝健脾方中、高剂量组能显著抑制小鼠血清ALT、AST活性的升高（ P＜0．01），抑制肝组织MDA含量升高（ P＜0．05或P＜0．01），提高肝组织SOD、GSH活性（ P＜0．05或P＜0．01），降低肝指数（ P＜0．05或P＜0．01），减轻肝脏的病理损伤。结论疏肝健脾方对小鼠化学性肝损伤具有一定的保护作用。
목적：연구소간건비방（ SGJP）대CCl4유발적화학간손상소서적보호작용。방법장소서수궤분위6조：정상대조조、모형조、소간건비방저제량조、소간건비방중제량조、소간건비방고제량조、양성약（련분쌍지， BP）조，급약조관위급약，매천1차，련속관위10 d，정상조화모형조용등량적용매，우말차급약8 h후，제정상조외기여매조복강주사0．1％CCl4감람유용액（0．1 mL/10 g）복제소서화학성간손상모형，검측소서혈청곡안산전안매（ALT）、천동안산전안매（AST）、간장지수、간조직초양화물기화매（SOD）、병이철（MDA）、곡광감태（GSH）함량，HE염색관찰간조직형태학변화。결과소간건비방중、고제량조능현저억제소서혈청ALT、AST활성적승고（ P＜0．01），억제간조직MDA함량승고（ P＜0．05혹P＜0．01），제고간조직SOD、GSH활성（ P＜0．05혹P＜0．01），강저간지수（ P＜0．05혹P＜0．01），감경간장적병리손상。결론소간건비방대소서화학성간손상구유일정적보호작용。
Objetc ive To study the protective effect of shuganjianpifang (SGJP) on carbon tetrachloride -induced chemical liver injury.Methods The mice are randomly divided into six groups: The Control, The model, The SGJP (7.8,15.6,31.2 g/kg),The BP.The SGJP were given by avage,1 times a day for 10 days.After being given drug for 8 hours, the Model were induced by injection of carbon 0.1%tetrachloride-olive oil solution (0.1 mL/10 g) by intra-peritoneal injection.After being given drug for 10 days, the ALT and the AST were assayed by spectrophotometry .The SOD, MDA,GSH were examined by Colorimetric methods .The liver index were also examined .The histopathologic chan-ges of the hepatic tissues were observed by the HE stained method .Results The liver index, the ALT level, the AST level and the MDA level were decreased by SGJP (15.6,31.2 g/kg).The SOD level and the GSH level were increased by SGJP.Moreover, the degree of the liver injury were also inhibited by SGJP .Conclu is ons SGJP is a significantly pro-tective drug in chemical liver injury mice .