CAJ | 학술논문

采用不同纯化方法制备大豆凝集素,其产率略有不同,其中采用N-乙酰基-D-半乳糖胺-Epoxy-Sepharose6B法制备产率最高,且N-乙酰基-D-半乳糖胺对大豆凝集素的亲和力较高,所制备的大豆凝集素活性测定与标准品一致,试验结果表明,该法是目前制备大豆凝集素的最优方法。
채용불동순화방법제비대두응집소,기산솔략유불동,기중채용N-을선기-D-반유당알-Epoxy-Sepharose6B법제비산솔최고,차N-을선기-D-반유당알대대두응집소적친화력교고,소제비적대두응집소활성측정여표준품일치,시험결과표명,해법시목전제비대두응집소적최우방법。
Different purification methods to prepare soybean agglutinin and its yield were slightly different, preparation production rate was the highest, when used the N acetyl D-galactose amine-Epoxy-Sepharose 6B and the N acetyl D-galactose amine on soybean agglutinin affinity is higher, the activity between the preparation of soybean agglutinin and standard had no different. The test results showed that the method was the best method for the preparation of soybean agglutinin.