CAJ | 학술논문

　　应用实时荧光定量PCR技术，通过室内营养钵土壤人工接种粉红粘帚霉67-1菌株，研究土壤温度、pH、含水率对生防菌粉红粘帚霉在土壤中消长动态的影响。结果表明：在不同温度、含水率和酸碱度的土壤中，菌株67-1种群动态变化曲线基本一致，接种5 d前土壤中67-1拷贝数明显减少，10 d后拷贝数逐渐增加，20 d时达到最高，并维持在这一水平。土壤因子对菌株67-1的定殖量具有显著影响，接种相同时间30℃时土壤中菌株67-1拷贝数增加最快，其次是20℃，10℃最低；土壤pH值为5.0时拷贝数最高， pH为8.0时拷贝数较低，pH为4.0时拷贝数最低；土壤含水率为15%时菌株67-1拷贝数高于含水率25%，但二者差异不显著，表明菌株67-1在土壤中的扩增受土壤含水率影响较小。
　　응용실시형광정량PCR기술，통과실내영양발토양인공접충분홍점추매67-1균주，연구토양온도、pH、함수솔대생방균분홍점추매재토양중소장동태적영향。결과표명：재불동온도、함수솔화산감도적토양중，균주67-1충군동태변화곡선기본일치，접충5 d전토양중67-1고패수명현감소，10 d후고패수축점증가，20 d시체도최고，병유지재저일수평。토양인자대균주67-1적정식량구유현저영향，접충상동시간30℃시토양중균주67-1고패수증가최쾌，기차시20℃，10℃최저；토양pH치위5.0시고패수최고， pH위8.0시고패수교저，pH위4.0시고패수최저；토양함수솔위15%시균주67-1고패수고우함수솔25%，단이자차이불현저，표명균주67-1재토양중적확증수토양함수솔영향교소。
By using real-time fluorescence quantitative PCR method, we conducted the effects of soil temperature, pH and moisture on population dynamics of Clonostachys rosea 67-1 in greenhouse. The results showed that the curves of population dynamics of 67-1 were consistent in different soil temperature, moisture and pH. The copy numbers of the fungus decreased obviously after inoculated for 5 days, and increased after 10 days. The greatest copy numbers were obtained in 20 days, and the fungal colonization maintained at the high level for a period of time. Soil factors had obvious effects on 67-1. The increasing rate of number of 67-1 copies was the highest under 30℃, followed by 20℃. The lowest amount of the biocontrol fungus was recovered in the soil treated under 10℃. When soil pH value was adjusted to 5.0, the copy number of 67-1was the highest, while the soil with pH 4.0 or pH 8.0 was not suitable for 67-1 proliferation. The copy numbers were higher in soil with the moisture of 25%than that with the moisture of 15%, however, there was no significant difference between them, which indicated that soil moisture did not significantly affecte colonization of C. rosea 67-1.