CAJ | 학술논문

目的：探讨颈椎后纵韧带骨化症（OPLL）与BMP-2基因第3外显子的rs79417223和BMP-4基因第4外显子的rs147822607两个SNP位点的相关性。方法选取47例浙江地区汉族颈椎后纵韧带骨化症患者，以100名正常个体作为对照组，采用PCR+Sanger DNA测序，统计分析2个位点基因型的分布信息。结果 BMP-2基因第3外显子的rs79417223G寅A患者、BMP-4第4外显子的rs147822607A寅C患者与正常组呈现明显差异（P约0.05，P约0.01）。结论 BMP-2基因第3外显子的rs79417223G寅A、BMP-4基因、第4外显子的rs147822607A寅C，这2个多态性位点与OPLL的发病具有强烈相关性。
목적：탐토경추후종인대골화증（OPLL）여BMP-2기인제3외현자적rs79417223화BMP-4기인제4외현자적rs147822607량개SNP위점적상관성。방법선취47례절강지구한족경추후종인대골화증환자，이100명정상개체작위대조조，채용PCR+Sanger DNA측서，통계분석2개위점기인형적분포신식。결과 BMP-2기인제3외현자적rs79417223G인A환자、BMP-4제4외현자적rs147822607A인C환자여정상조정현명현차이（P약0.05，P약0.01）。결론 BMP-2기인제3외현자적rs79417223G인A、BMP-4기인、제4외현자적rs147822607A인C，저2개다태성위점여OPLL적발병구유강렬상관성。
Objective To explore the relationship between 2 single nucleotide polymorphisms(SNPs) of BMP-2 and BMP-4 and ossification of posterior longitudinal ligament of the spine(OPLL)in a Chinese population. Methods Forty-seven sporadic OPLL patients and 100 healthy controls in Zhejiang area were recruited for association analy-sis. The SNP regions of BMP-2 and BMP-4 genes were amplified by PCR and Sanger sequencing. Genotype fre-quencies of the SNPs were compared between the cases and control subjects with SPSS 13.0 software. Results Both Of the rs79417223 G→A site in the exon 3 of BMP-2 and the rs147822607 A→C site in the exon 4 of BMP-4,the patient and normal control groups showed significant differences(P<0.05,P<0.01). Conclusions The rs79417223 and rs147822607 have a strong correlation to OPLL.