四川畜牧兽医
四川畜牧獸醫
사천축목수의
SICHUAN ANIMAL & VETERINARY SCIENCES
2012年
10期
25-27
,共3页
董高华%徐怀亮%姚永芳%黄晓峰%蒋美山
董高華%徐懷亮%姚永芳%黃曉峰%蔣美山
동고화%서부량%요영방%황효봉%장미산
遗传多样性%野猪%外显子2
遺傳多樣性%野豬%外顯子2
유전다양성%야저%외현자2
Genetic diversity%Wild boar%Exon 2
本试验采用DNA测序技术测定了15头野猪235bp的SLA—DQB基因外显子2序列.结合GeneBank中报道的另3头野猪和18头家猪共36个个体的相应序列,重点对野猪SLA—DQB外显子2进行核苷酸多态性分析。结果表明野猪SLA—DQB外显子2具有较丰富的核苷酸多态性;在家猪品种改良中,野猪具有潜在的遗传价值。
本試驗採用DNA測序技術測定瞭15頭野豬235bp的SLA—DQB基因外顯子2序列.結閤GeneBank中報道的另3頭野豬和18頭傢豬共36箇箇體的相應序列,重點對野豬SLA—DQB外顯子2進行覈苷痠多態性分析。結果錶明野豬SLA—DQB外顯子2具有較豐富的覈苷痠多態性;在傢豬品種改良中,野豬具有潛在的遺傳價值。
본시험채용DNA측서기술측정료15두야저235bp적SLA—DQB기인외현자2서렬.결합GeneBank중보도적령3두야저화18두가저공36개개체적상응서렬,중점대야저SLA—DQB외현자2진행핵감산다태성분석。결과표명야저SLA—DQB외현자2구유교봉부적핵감산다태성;재가저품충개량중,야저구유잠재적유전개치。
In this study, the partial exon 2 of SLA-DQB gene was amplified and sequencing, a uniform fragment of 235 bp was obtained in 15 wild pigs. Combining 15 tested sequences with 3 wild boars and 18 domestic pigs published sequences from GeneBank, the nueleotide diversity in wild boar was analysed. The results shown in this study indicated that the SLA-DQB gene of wild boar were highly polymorphic, the potential genetic value of wild boar could be used to improve the productive traits of the domestic pigs.