现代农业科技
現代農業科技
현대농업과기
XIANDAIHUA NONGYE
2012年
18期
295-296,298
,共3页
高效液相色谱—串联质谱%韭菜%有机磷%残留%测定
高效液相色譜—串聯質譜%韭菜%有機燐%殘留%測定
고효액상색보—천련질보%구채%유궤린%잔류%측정
high performance liquid chromatography-tandem mass spectrometry%leek%organophosphorus pesticides%residue%determination
采用高效液相色谱—串联质谱法测定韭菜中12种有机磷农药多残留。样品通过乙腈提取,Sep-pak Carbon NH2固相萃取柱净化,Venusil MP C18色谱柱分离,电喷雾正离子源（ESI＋）扫描,多反应监测模式（MRM）测定。结果表明：12种有机磷农药的检出限为0.02～0.60μg/kg,在10.0、20.0、50.0μg/kg 3个加标水平时,平均回收率为70%～95%。
採用高效液相色譜—串聯質譜法測定韭菜中12種有機燐農藥多殘留。樣品通過乙腈提取,Sep-pak Carbon NH2固相萃取柱淨化,Venusil MP C18色譜柱分離,電噴霧正離子源（ESI＋）掃描,多反應鑑測模式（MRM）測定。結果錶明：12種有機燐農藥的檢齣限為0.02～0.60μg/kg,在10.0、20.0、50.0μg/kg 3箇加標水平時,平均迴收率為70%～95%。
채용고효액상색보—천련질보법측정구채중12충유궤린농약다잔류。양품통과을정제취,Sep-pak Carbon NH2고상췌취주정화,Venusil MP C18색보주분리,전분무정리자원（ESI＋）소묘,다반응감측모식（MRM）측정。결과표명：12충유궤린농약적검출한위0.02～0.60μg/kg,재10.0、20.0、50.0μg/kg 3개가표수평시,평균회수솔위70%～95%。
A HPLC-MS/MS method was established for the determination of 12 kinds of organophosphorus pesticides residues in leek .The samples was extracted by acetonitrile ,cleaned up with Sep-pak Carbon NH2 Solid phase extraction column ,the analytes was separated on Venusil MP Cls column, indentified under positive clcetrospray ionization (ESI+) ,multiple reaction monitoring (MRM)mode. The LOD of 12 kinds of organophosp- horus pesticides was in 0.02-0.60 p,g,/kg, the recoveries at three spiked concentration levels of 10.0,20.0 and 50.0 p,g/kg ranged from 70% to 95%.
