CAJ | 학술논문

以彩叶草节段为外植体进行离体培养,对外植体灭菌方法以及培养基中不同种类外源激素的浓度对试管苗增殖、生根的影响等进行了研究。结果表明：以0.1%HgCl2灭菌8 min,无菌外植体成活率可以达到70%;在MS＋6-BA 2.0 mg/L＋NAA 0.20 mg/L的增殖培养基上培养30 d,试管苗的增殖系数可达8.8;在1/2MS＋6-BA 0.2 mg/L＋NAA 0.5 mg/L的生根培养基上培养21 d后,生根率为100.0%,生根5.6条/株;炼苗后移植成活率达95%。
이채협초절단위외식체진행리체배양,대외식체멸균방법이급배양기중불동충류외원격소적농도대시관묘증식、생근적영향등진행료연구。결과표명：이0.1%HgCl2멸균8 min,무균외식체성활솔가이체도70%;재MS＋6-BA 2.0 mg/L＋NAA 0.20 mg/L적증식배양기상배양30 d,시관묘적증식계수가체8.8;재1/2MS＋6-BA 0.2 mg/L＋NAA 0.5 mg/L적생근배양기상배양21 d후,생근솔위100.0%,생근5.6조/주;련묘후이식성활솔체95%。
The in vitro culture of Coleus blumei was established by using the nodal stem segments as explants. The method of surface sterilization of explants ,the effects of different plant growth regulators on the muhiplication and rooting were investigated. The results showed that 70% sterile explants could be obtained by surface treatment with 0.1% HgC12 for 8 minutes.When cultured on a MS multiplication medium supplemented with 2.0 mg/L of 6-BA and 0.2 mg/L of NAA for 30 d, the test-tube seedling exhibited a multiplication coefficient of 8.8. For optimal rooting, induction on a 1/2 MS rooting medium supplemented with 0.2 mg/L of 6-BA and 0.5 mg/L of NAA for 21 days could produce rooted plantlets and each plantlet generated 5.6 roots on average. Rooted plantlets were acclimatized and transferred into outdoor environment with 95% survival rate.