CAJ | 학술논문

建立了滇桂艾纳香片中原儿茶酸的含量测定方法。用岛津VP-ODS色谱柱（150×4.6 mm,5μm）;甲醇-1%冰醋酸溶液（7∶93）为流动相;检测波长为256 nm,流速1.0 mL·min^-1。结果表明：原儿茶酸在0.041～0.329μg范围内与峰面积呈良好线性关系,r=0.9999,原儿茶酸平均加样回收率为98.55%,RSD为1.04%,精密度、稳定性良好。本方法可靠性高,分离度、准确性、重复性好,适用于滇桂艾纳香片中原儿茶酸的含量测定。
건립료전계애납향편중원인다산적함량측정방법。용도진VP-ODS색보주（150×4.6 mm,5μm）;갑순-1%빙작산용액（7∶93）위류동상;검측파장위256 nm,류속1.0 mL·min^-1。결과표명：원인다산재0.041～0.329μg범위내여봉면적정량호선성관계,r=0.9999,원인다산평균가양회수솔위98.55%,RSD위1.04%,정밀도、은정성량호。본방법가고성고,분리도、준학성、중복성호,괄용우전계애납향편중원인다산적함량측정。
To develop an HPLC method for determination of protocatochuic acid in Diangui' ainaxiang Pian,Shimadzu VP-ODS(4.6 mm×150 mm,5 μm)was used as chromatographic column.The mobile phase was a mixture of methanol-1% acetic acid(10:90),the flow-rate was 1 mL · min^-1.The detector wavelength was 256 nm.The results showed that the calibration curve was linear over the range of 0.041~0.329 μg for protocatochuic acid(r=0.9999),the average recovery of protocatochuic acid was 98.55%.The precision and stability were fine.The proposed method may be used in the quantitative analysis of protocatochuic acid in Diangui' ainaxiang Pian with high reliability,separation,accuracy,repeatability