CAJ | 학술논문

利用单因素和正交试验法研究了蛋白核小球藻（Chlorella pyrenoidos aFACHB-29）培养基中的碳源、氮源、碳氮比、pH、温度等对其生物量和脂肪含量的影响。首先用单因素方法对5个相关影响因素的效应进行了评价，并筛选出了对生物量生长和脂肪含量有显著影响的3个因素：葡萄糖浓度、硝酸铵浓度和pH；再用正交试验法确定了生物量生长的最佳条件为：葡萄糖浓度为2％，pH值为7．5，硝酸铵浓度为0．016％。最终，发酵液中的藻体含量可达到5．6g，L，脂肪含量可达到1．46g/L。
이용단인소화정교시험법연구료단백핵소구조（Chlorella pyrenoidos aFACHB-29）배양기중적탄원、담원、탄담비、pH、온도등대기생물량화지방함량적영향。수선용단인소방법대5개상관영향인소적효응진행료평개，병사선출료대생물량생장화지방함량유현저영향적3개인소：포도당농도、초산안농도화pH；재용정교시험법학정료생물량생장적최가조건위：포도당농도위2％，pH치위7．5，초산안농도위0．016％。최종，발효액중적조체함량가체도5．6g，L，지방함량가체도1．46g/L。
The effects of carbon source, nitrogen source, carbon-nitrogen ration, pH value and temperature on the biomass and lipid concentration were studied in the fermentation condition of Chlorella pyrenoidosa FACHB-29 by the single factor and orthogonal experiments. First, the single factor was used to evaluate the influence of the related factors. Then, the three factorsaffecting the biomass and lipid concentration notably were screened out as glucose concentration, ammonium nitrate concentration and pH value. Finally, the optimum conditions for the cell growth were determined through the orthogonal test, that is, 2% glucose concentration, 0.016% ammonium nitrate concentration, and pH value 7.5. Under the optimum conditions, the Chlorella pyrenoidosa cell concentration reached 5.6 g/L in the fermentation, and the lipid yield reached 1.46 g/L.