CAJ | 학술논문

为研究玉柏石松提取物山芝烯二醇对体外培养小鼠颅骨成骨细胞活性的影响,采用MTT法、碱性磷酸酶（ALP）试剂盒、荧光定量PCR检测不同浓度药物作用不同时间后成骨细胞增殖、ALP活性和成骨活性相关基因,如原癌基因（c-jun、c-fos）、成骨转录因子（Osterix）、碱性磷酸酶（ALP）、Ⅰ型胶原（Col-Ⅰ）、骨钙素（OC）表达.结果显示：山芝烯二醇处理早期（3 d）促进成骨细胞增殖,促进c-jun、c-fos基因表达,先抑制后促进Osterix基因表达;晚期（9 d）促进ALP活性和ALP、Col-Ⅰ基因表达,对OC基因无明显影响.说明山芝烯二醇可以促进成骨细胞的增值,ALP活性及部分骨相关基因表达,是玉柏石松促进骨愈合的有效成分之一.
위연구옥백석송제취물산지희이순대체외배양소서로골성골세포활성적영향,채용MTT법、감성린산매（ALP）시제합、형광정량PCR검측불동농도약물작용불동시간후성골세포증식、ALP활성화성골활성상관기인,여원암기인（c-jun、c-fos）、성골전록인자（Osterix）、감성린산매（ALP）、Ⅰ형효원（Col-Ⅰ）、골개소（OC）표체.결과현시：산지희이순처리조기（3 d）촉진성골세포증식,촉진c-jun、c-fos기인표체,선억제후촉진Osterix기인표체;만기（9 d）촉진ALP활성화ALP、Col-Ⅰ기인표체,대OC기인무명현영향.설명산지희이순가이촉진성골세포적증치,ALP활성급부분골상관기인표체,시옥백석송촉진골유합적유효성분지일.
To study the effect of serratenediol(an extract from Lycopodium obscurum L.) on the osteogenic activity of cultured osteoblasts,MTT assay、 alkaline phosphate(ALP) assay kit and real-time PCR were used to determine the proliferation rate,ALP activity and osteogenic genes(c-jun、c-fos、Osterix、ALP、Col-Ⅰ、OC) expression of osteoblasts.The results elucidated that treatment of serratenediol for 3 days could promote the proliferation rate,the expression of c-jun and c-fos genes and first inhibit then enhance Osterix gene of osteoblasts.Serratencdiol treatment for 9 days could enhance the expression of ALP and Col-Ⅰ genes.There was no obvious effect of serratenediol on the expression of OC.So serratenediol could promote the proliferation rate,ALP activity and some osteogenic gene expression of osteoblasts.It is one of the effective component from Lycopodium obscurum L.for bone healing.