仲恺农业工程学院学报
仲愷農業工程學院學報
중개농업공정학원학보
JOURNAL OF ZHONGKAI UNIVERSITY OF AGRICULTURE AND TECHNOLOGY
2012年
3期
1-5
,共5页
刘文%杜贵峰%陈基成%陈天才%梁红
劉文%杜貴峰%陳基成%陳天纔%樑紅
류문%두귀봉%진기성%진천재%량홍
中华猕猴桃(Actinidia%chinensis%Planch.)%RAPD%性别相关标记%性别鉴定
中華獼猴桃(Actinidia%chinensis%Planch.)%RAPD%性彆相關標記%性彆鑒定
중화미후도(Actinidia%chinensis%Planch.)%RAPD%성별상관표기%성별감정
Actinidia chinensis%RAPD%sex-related marker%sex identification
为寻找一种适合于中华猕猴桃(Actinidia chinensisPlanch.)的性别分子标记,以广东省和平县主栽中华猕猴桃的4个雌株品种、4个雄株品种以及“和平红阳”品种种子实生苗为试材,通过基因组DNA提取、RAPD扩增和比较分析,筛选出了6个带型清晰、雌雄性别差异明显的RAPD引物,并从6个引物扩增产物中分离出“和平红阳”种子实生苗雌性特征片段$1044—900和S1044—1350;另外用引物S1032对8个栽培品种雌雄植株基因组DNA扩增后发现了3个与雄性连锁的DNA标记,其中1条片段为NCBIGeneBank收录的猕猴桃雄性链锁标记S1032—850(登陆号:AY336259).根据NCBI Gene Bank收录的S1032—850序列设计特异性引物对4个雌株品种和4个雄株品种的基因组DNA扩增后发现,此标记在雄株中均有出现,在雌株未出现.
為尋找一種適閤于中華獼猴桃(Actinidia chinensisPlanch.)的性彆分子標記,以廣東省和平縣主栽中華獼猴桃的4箇雌株品種、4箇雄株品種以及“和平紅暘”品種種子實生苗為試材,通過基因組DNA提取、RAPD擴增和比較分析,篩選齣瞭6箇帶型清晰、雌雄性彆差異明顯的RAPD引物,併從6箇引物擴增產物中分離齣“和平紅暘”種子實生苗雌性特徵片段$1044—900和S1044—1350;另外用引物S1032對8箇栽培品種雌雄植株基因組DNA擴增後髮現瞭3箇與雄性連鎖的DNA標記,其中1條片段為NCBIGeneBank收錄的獼猴桃雄性鏈鎖標記S1032—850(登陸號:AY336259).根據NCBI Gene Bank收錄的S1032—850序列設計特異性引物對4箇雌株品種和4箇雄株品種的基因組DNA擴增後髮現,此標記在雄株中均有齣現,在雌株未齣現.
위심조일충괄합우중화미후도(Actinidia chinensisPlanch.)적성별분자표기,이광동성화평현주재중화미후도적4개자주품충、4개웅주품충이급“화평홍양”품충충자실생묘위시재,통과기인조DNA제취、RAPD확증화비교분석,사선출료6개대형청석、자웅성별차이명현적RAPD인물,병종6개인물확증산물중분리출“화평홍양”충자실생묘자성특정편단$1044—900화S1044—1350;령외용인물S1032대8개재배품충자웅식주기인조DNA확증후발현료3개여웅성련쇄적DNA표기,기중1조편단위NCBIGeneBank수록적미후도웅성련쇄표기S1032—850(등륙호:AY336259).근거NCBI Gene Bank수록적S1032—850서렬설계특이성인물대4개자주품충화4개웅주품충적기인조DNA확증후발현,차표기재웅주중균유출현,재자주미출현.
To find a sex-related marker that could be used to identify leaves from 4 cuhivars of male, 4 cuhivars of female and ' Hongyang' the sex of Actinidia chinensis, young seedling kiwifruit plants (Actinidia chinensis) were used to extract their genomic DNA. RAPD amplification was carried out with 20 random primers, from which 6 RAPD primers were selected according to the RAPD differences between male and female plants. Two female-related markers S1044-900 and S1044-1350 were isolated based on their amplification results in all the ' Hongyang' female seedlings,but not in male ones, when the primer S1044 was used in the RAPD amplification. Three male-related markers were found according to the RAPD amplification result with the primer S1032, one of which was the S1032-850 marker included in NCBI database( GenBank accession no.:AY336259). PCR amplification of the DNA samples with a specific primer pair based on S1032-850 sequence showed that the S1032-850 fragment was amplified in all 4 cuhivars male plants, but not in female plants.