CAJ | 학술논문

以5＇-C6-T0-A1,5＇-C6-T5-A1和5＇-C6-T10-A1三条不同长度连接臂修饰的凝血酶适体作为生物传感器的识别分子,结合巯基自组装技术,利用电化学阻抗技术,测定了凝血酶的线性范围和检出限,比较了三者灵敏度的差异。利用浓度平衡法计算了适体与凝血酶作用的结合常数,三条链的结合常数Ka分别为0.32 nM-1,0.84 nM-1和0.67 nM-1,表明,5＇-C6-T5-A1组装的传感器与凝血酶的结合常数最大,灵敏度最好。
이5＇-C6-T0-A1,5＇-C6-T5-A1화5＇-C6-T10-A1삼조불동장도련접비수식적응혈매괄체작위생물전감기적식별분자,결합구기자조장기술,이용전화학조항기술,측정료응혈매적선성범위화검출한,비교료삼자령민도적차이。이용농도평형법계산료괄체여응혈매작용적결합상수,삼조련적결합상수Ka분별위0.32 nM-1,0.84 nM-1화0.67 nM-1,표명,5＇-C6-T5-A1조장적전감기여응혈매적결합상수최대,령민도최호。
The aptamer-thrombin interaction effect for the linker structure was studied using electrochemical impedance spectroscopy.Three linker structure aptamers,5'-C6-T0-A1,5'-C6-T5-A1 and 5'-C6-T10-A1,were immobilized on a gold electrode by the self-assembled technique.The linear range and the detection limit of the three aptasensors were obtained.The association constants Ka of the binding reaction of three aptamers with interaction of thrombin,were determined according to concentration equilibrium.The results showed that.association constants Ka is 0.32 nM-1,0.84 nM-1 and 0.67 nM-1respectively.