徐州工程学院学报:自然科学版
徐州工程學院學報:自然科學版
서주공정학원학보:자연과학판
Journal of Xuzhou Istitute of Technology
2012年
3期
46-50
,共5页
新诺明%水丝蚓%半致死质量浓度%超氧化物歧化酶%过氧化物酶
新諾明%水絲蚓%半緻死質量濃度%超氧化物歧化酶%過氧化物酶
신낙명%수사인%반치사질량농도%초양화물기화매%과양화물매
sulfamethoxazole%Limnodilus hoffineisteri%semi-lethal concentration(LC50)%superoxide dismutase(SOD)%peroxidase(POD)
采用急性毒性方法测定了被不同质量浓度新诺明处理的水丝蚓半致死质量浓度(LC50),利用测定酶活性方法检测了中毒后水丝蚓超氧化物歧化酶(SOD)和过氧化物酶(POD)的变化.结果表明:新诺明溶液对水丝蚓48hLC50为1.4263g/L;新诺明对水丝蚓SOD和POD活性影响非常明显(P〈0.01),当新诺明质量浓度小于1.4125g/L时,SOD、POD活性呈现上升趋势,而质量浓度大于1.4125g/L时,则酶活性呈现下降趋势;经1.4263g/L(LC50)新诺明处理后存活的水丝蚓,恢复过程中SOD、POD活性均呈现下降趋势,72h后,二种酶活性接近于正常动物体酶活性水平.
採用急性毒性方法測定瞭被不同質量濃度新諾明處理的水絲蚓半緻死質量濃度(LC50),利用測定酶活性方法檢測瞭中毒後水絲蚓超氧化物歧化酶(SOD)和過氧化物酶(POD)的變化.結果錶明:新諾明溶液對水絲蚓48hLC50為1.4263g/L;新諾明對水絲蚓SOD和POD活性影響非常明顯(P〈0.01),噹新諾明質量濃度小于1.4125g/L時,SOD、POD活性呈現上升趨勢,而質量濃度大于1.4125g/L時,則酶活性呈現下降趨勢;經1.4263g/L(LC50)新諾明處理後存活的水絲蚓,恢複過程中SOD、POD活性均呈現下降趨勢,72h後,二種酶活性接近于正常動物體酶活性水平.
채용급성독성방법측정료피불동질량농도신낙명처리적수사인반치사질량농도(LC50),이용측정매활성방법검측료중독후수사인초양화물기화매(SOD)화과양화물매(POD)적변화.결과표명:신낙명용액대수사인48hLC50위1.4263g/L;신낙명대수사인SOD화POD활성영향비상명현(P〈0.01),당신낙명질량농도소우1.4125g/L시,SOD、POD활성정현상승추세,이질량농도대우1.4125g/L시,칙매활성정현하강추세;경1.4263g/L(LC50)신낙명처리후존활적수사인,회복과정중SOD、POD활성균정현하강추세,72h후,이충매활성접근우정상동물체매활성수평.
We determined the LC50 by dealing Limnodilus hoffineisteri with sulfamethoxazole of dif- ferent concentrations, and then determined the SOD and POD activity of poisoned Limnodilus hoffi- neisteri. The experiment shows : the LC50 of Limnodilus hoffineisteri dealt with sulfamethoxazole in 48 h is 1. 4263g/L; the sulfamethoxazole has a conspicuous influence on the activity of SOD and POD, and the SOD and POD activity of Limnodilus hoffineisteri which is dealt with different concentrations of sulfame- thoxazole increase when the concentration is below 1. 412 5 g/L and decline when over 1. 412 5 g/L; the SOD and POD activity of Limnodilus hoffineisteri which is dealt with 1. 426 3 g/L(LC50)sulfamethox- azole first increase then decline, and return to the normal level after 72 h.