沈阳化工大学学报
瀋暘化工大學學報
침양화공대학학보
JOURNAL OF SHENYANG INSTITUTE OF CHEMICAL TECHNOLOGY
2012年
3期
215-218,274
,共5页
筛选%蓝色素%优化%色价
篩選%藍色素%優化%色價
사선%람색소%우화%색개
screening cyanine optimization colourity
从土壤中筛选到1株蓝色素产量较高的放线菌,命名为Cya-5.对蓝色素发酵条件进行研究,结果显示发酵培养基的配方为甘油20 g/L、KNO31 g/L,K2HPO40.5 g/L,MgSO4.7H2O 0.5 g/L,FeSO4.7H2O 0.01 g/L,NaCl 0.5 g/L,pH=8.5,装液量为100 mL/250 mL三角瓶、30℃发酵96 h,发酵液的A590总值达到12.02.利用碱沉法提取发酵液中的色素物质,粗提物色价可达到45.8.
從土壤中篩選到1株藍色素產量較高的放線菌,命名為Cya-5.對藍色素髮酵條件進行研究,結果顯示髮酵培養基的配方為甘油20 g/L、KNO31 g/L,K2HPO40.5 g/L,MgSO4.7H2O 0.5 g/L,FeSO4.7H2O 0.01 g/L,NaCl 0.5 g/L,pH=8.5,裝液量為100 mL/250 mL三角瓶、30℃髮酵96 h,髮酵液的A590總值達到12.02.利用堿沉法提取髮酵液中的色素物質,粗提物色價可達到45.8.
종토양중사선도1주람색소산량교고적방선균,명명위Cya-5.대람색소발효조건진행연구,결과현시발효배양기적배방위감유20 g/L、KNO31 g/L,K2HPO40.5 g/L,MgSO4.7H2O 0.5 g/L,FeSO4.7H2O 0.01 g/L,NaCl 0.5 g/L,pH=8.5,장액량위100 mL/250 mL삼각병、30℃발효96 h,발효액적A590총치체도12.02.이용감침법제취발효액중적색소물질,조제물색개가체도45.8.
One strain of actinomyces which can produce cyanine is screened,and named Cya-5.The conditions of cyanine fermentation have been studied,and the results show that the suitable formula of medium is that glycerin 20 g/L,KNO3 1 g/L,K2HPO4 0.5 g/L,MgSO4 · 7 H2O 0.5 g/L,FeSO4 · 7 H2O 0.01 g/L,NaCl 0.5 g/L,pH=8.5,100 mL/250 mL triangle bottle,temperature 30 ℃ fermentation 96 h,and A590 of fermented liquid is 1.202.The crude extracting pigment is obtained by alkali sink method,and its colourity is 45.8.
