CAJ | 학술논문

本文采用磁珠富集法筛选新疆扁吻鱼（Aspiorhynchus laticeps Day）的微卫星分子标记,获得13个位点,同时随机抽取1000对鲤引物检测扁吻鱼同源位点的多态性,获得具多态性位点18对。应用31对引物对扁吻鱼与塔里木裂腹鱼（Schizthorax biddulphi）进行遗传多样性分析,共检测到110个等位基因,每个位点的等位基因数为2~6个,扁吻鱼群体平均多态信息含量为0.5353,平均观测杂合度为0.5306,平均期望杂合度为0.6057。表明扁吻鱼群体多态性较高,遗传多样性处于中等偏上水平。群体间遗传距离为0.4763,遗传相似系数为0.6211,表明两个群体为同科不同属的群体。
본문채용자주부집법사선신강편문어（Aspiorhynchus laticeps Day）적미위성분자표기,획득13개위점,동시수궤추취1000대리인물검측편문어동원위점적다태성,획득구다태성위점18대。응용31대인물대편문어여탑리목렬복어（Schizthorax biddulphi）진행유전다양성분석,공검측도110개등위기인,매개위점적등위기인수위2~6개,편문어군체평균다태신식함량위0.5353,평균관측잡합도위0.5306,평균기망잡합도위0.6057。표명편문어군체다태성교고,유전다양성처우중등편상수평。군체간유전거리위0.4763,유전상사계수위0.6211,표명량개군체위동과불동속적군체。
In this study,magnetic beads enriched method was used to isolate microsatellite DNA from Aspiorhynchus laticeps Day genome and 13 sites were found.One thousand pairs of primers from common carp Cyprinus carpio randomly were chosen to test for the cross-species amplification in Aspiorhynchus laticeps Day,and 18 primers were amplified successfully.Polymorphism of all these 31 distinct loci was assessed using 24 Aspiorhynchus laticeps Day individuals and 19 Schizthorax biddulphi Gvnther,and a total of 110 alleles were detected.The Aspiorhynchus laticeps Day was shown to have average PIC of 0.5353,Ho of 0.5306,and He of 0.6057,indicating that the polymorphism of Aspiorhynchus laticeps groups is high and the genetic diversity is in the mid to upper level.The Aspiorhynchus laticeps Day and Schizthorax biddulphi Gvnther were found to show genetic distance of 0.6211 and genetic similarity coefficient of 0.4763,indicating that the two groups is of different genera in the same family.