CAJ | 학술논문

作为两种较常见的抗癌药物，紫杉醇与白藜芦醇在临床试验中均证实具有良好的抗癌效果．本文意在研究紫杉醇与白藜芦醇单独及联合用药时对人肝癌细胞HepG-2活性的影响，探索两种药物联合应用时的药效变化．实验中通过CCK．8法测定了不同浓度的紫杉醇和白藜芦醇单独及联合用药时对人肝癌细胞HepC-2的活性影响，计算出其抑制率和半抑制率浓度，并通过金氏修正公式进行药效判定．结果发现当紫杉醇和白藜芦醇单独用药时，两者对HepG-2细胞的生长均有较高的抑制作用，在设定的药物浓度范围内，前后两者的抑制率分别达到了45．81％-81．80％和34．43％-93.60％，并且呈现出明显的剂量依赖性．当二者联合作用时，紫杉醇与白藜芦醇分别在在5—20umol／L以及100—1000umol／L的浓度范围内呈现出相加效果，且药品的半抑率制浓度均出现不同程度的降低，分别由单独用药时的1．47和122．07umol／L下降至联合作用时的0．65和42．41umol／L．据此判断，紫杉醇与白藜芦醇联合作用确实可显著增强药物对HepG-2细胞的抑制作用．二者具有相加抗肿瘤效果．
작위량충교상견적항암약물，자삼순여백려호순재림상시험중균증실구유량호적항암효과．본문의재연구자삼순여백려호순단독급연합용약시대인간암세포HepG-2활성적영향，탐색량충약물연합응용시적약효변화．실험중통과CCK．8법측정료불동농도적자삼순화백려호순단독급연합용약시대인간암세포HepC-2적활성영향，계산출기억제솔화반억제솔농도，병통과금씨수정공식진행약효판정．결과발현당자삼순화백려호순단독용약시，량자대HepG-2세포적생장균유교고적억제작용，재설정적약물농도범위내，전후량자적억제솔분별체도료45．81％-81．80％화34．43％-93.60％，병차정현출명현적제량의뢰성．당이자연합작용시，자삼순여백려호순분별재재5—20umol／L이급100—1000umol／L적농도범위내정현출상가효과，차약품적반억솔제농도균출현불동정도적강저，분별유단독용약시적1．47화122．07umol／L하강지연합작용시적0．65화42．41umol／L．거차판단，자삼순여백려호순연합작용학실가현저증강약물대HepG-2세포적억제작용．이자구유상가항종류효과．
Paclitaxel and resveratrol are two common anti-cancer drugs that have proved effective in clinical trials. In this study, we investigated the independent effect of the two drugs as well as their combined effect on the viability of HepG-2 cells, trying to elucidate the joint effect of the two drugs. The independent and combined effects of the two drugs on HepG-2 at various concentrations were measured by CCK-8 assay; inhibitory rates and IC50 were calculated and efficacies were judged according to the Revised Jin's Formula. Both paclitaxel and resveratrol showed strong inhibitory effect on HepG-2 cells and at the tested concentration the inhibitory rates were up to 45.81% - 81.80% and 34.43% -96.30% respectively, showing an obvious dose-dependency. The two drugs showed combined effects when used together at the concentration of 5 -20 umol/L for paclitaxel and 100 - 1000umol/L for resveratrol. And their IC50 were also decreased from 1. 47umol/L and 122.07umol/L when used independently to 0. 65umoL/L and 42.41 umol/L when used together. Our results confirmed that combination use of paclitaxel and resveratrol can significantly enhance their anti-cancer effect, that is to say, the two drugs have synergy effect.