CAJ | 학술논문

从厌氧消化池活性污泥中采集样品，利用特定培养基进行厌氧培养，分离筛选得到2株菌，分别编号为A、B．通过特定荧光反应检测鉴定A、B均为产甲烷菌．利用甲醇液体培养基对A、B两种菌株进行培养，20d后两株菌均产生甲烷，且通过自制气体收集装置测定其产气量发现，B菌株的产气量高于A菌株．
종염양소화지활성오니중채집양품，이용특정배양기진행염양배양，분리사선득도2주균，분별편호위A、B．통과특정형광반응검측감정A、B균위산갑완균．이용갑순액체배양기대A、B량충균주진행배양，20d후량주균균산생갑완，차통과자제기체수집장치측정기산기량발현，B균주적산기량고우A균주．
Two strains of Methanogen were isolated from anaerobic digester by anaerobic culture, and identified by the specific fluoroscopy. They were marked as strain A and strain B. A gas collector was designed to collect the methane which was produced by strain A or B. The results showed that strain A and B both could produce gas after about 20 days cultivation with methanol liquid medium, and the gas production of B strain was more than A strain' s.