湖北民族学院学报：自然科学版
湖北民族學院學報：自然科學版
호북민족학원학보：자연과학판
Journal of Hubei Institute for Nationalities(Natural Sciences)
2012年
3期
335-338
,共4页
曾智%江巍%田瑞%武芸%董静洲%郑小江
曾智%江巍%田瑞%武蕓%董靜洲%鄭小江
증지%강외%전서%무예%동정주%정소강
海金沙%孢子叶%GGB%分化
海金沙%孢子葉%GGB%分化
해금사%포자협%GGB%분화
Lygodium japonicum%spore leaves%GGB%differentiate
以海金沙孢子叶为材料进行了繁殖研究,得出孢子叶分化为GGB的最佳条件为MS＋NAA 0.3mg/L＋6-BA0.5 mg/L,诱导率达90%;GGB增殖分化形成幼苗的最佳条件为MS＋6-BA1.5mg/＋NaH_2PO_4200mg/L,增值系数达7.8;生根培养基最佳条件为1/2 MS＋NAA 0.1 mg/L＋2%蔗糖,生根率达86.7%或者是1/2 MS＋IBA0.2 mg/L＋2%蔗糖生根率为87%;炼苗移栽到珍珠岩：草炭=2：1的基质中,成活率达87.5%.能在短期内生产出大量海金沙组培苗,为工厂化育苗提供依据.
以海金沙孢子葉為材料進行瞭繁殖研究,得齣孢子葉分化為GGB的最佳條件為MS＋NAA 0.3mg/L＋6-BA0.5 mg/L,誘導率達90%;GGB增殖分化形成幼苗的最佳條件為MS＋6-BA1.5mg/＋NaH_2PO_4200mg/L,增值繫數達7.8;生根培養基最佳條件為1/2 MS＋NAA 0.1 mg/L＋2%蔗糖,生根率達86.7%或者是1/2 MS＋IBA0.2 mg/L＋2%蔗糖生根率為87%;煉苗移栽到珍珠巖：草炭=2：1的基質中,成活率達87.5%.能在短期內生產齣大量海金沙組培苗,為工廠化育苗提供依據.
이해금사포자협위재료진행료번식연구,득출포자협분화위GGB적최가조건위MS＋NAA 0.3mg/L＋6-BA0.5 mg/L,유도솔체90%;GGB증식분화형성유묘적최가조건위MS＋6-BA1.5mg/＋NaH_2PO_4200mg/L,증치계수체7.8;생근배양기최가조건위1/2 MS＋NAA 0.1 mg/L＋2%자당,생근솔체86.7%혹자시1/2 MS＋IBA0.2 mg/L＋2%자당생근솔위87%;련묘이재도진주암：초탄=2：1적기질중,성활솔체87.5%.능재단기내생산출대량해금사조배묘,위공엄화육묘제공의거.
In this paper,Lygodium spore leaves were selected as the propagation materials.The optimum condition for the sporophyll differentiated into GGB was MS+NAA0.3 mg / L+6-BA0.5 mg/L,and the induction rate was 90%;GGB proliferation and differentiation of seedlings of the optimum conditions for the formation is MS+6-BA1.5 mg/+NaH_2PO_4200 mg/L,and the value-added factor is 7.8;the best conditions for rooting is 1/2 MS+NAA0.1 mg/L+2%sucrose with the rooting rate at 86.7%or 1/2 MS +IBA0.2 mg/L+2%sucrose with the rooting rate at 87%;After the seedlings were rooted of regenerating shoots cultured,Lygodium was moved into the ratio of perlite and peat is 2:1.The survival rate was 95%,so we can produce a large number of Lygodium tissue culture seedlings in a short term and provide the basis for large-scale breeding.
