水稻转录因子WRKY39的克隆表达及DNA结合活性分析
수도전록인자WRKY39적극륭표체급DNA결합활성분석
Cloning and Expression of a Rice WRKY39 Gene and DNA-binding Analvsis
  • 万方数据
    广西科学院学报 엄서과학원학보
    JOURNAL OF GUANGXI ACADEMY OF SCIENCES
    2012年 3期 197-200 ,共4页

    郑元涛%欧娜%韦星明

    정원도%구나%위성명

    转录因子%WRKY%水稻%原核表达%DNA结合活性 轉錄因子%WRKY%水稻%原覈錶達%DNA結閤活性
    전록인자%WRKY%수도%원핵표체%DNA결합활성
    transcriptional factor%WRKY%Oryza sativa%prokaryotic expression%DNA-bind-ing activity
    根据水稻转录因子WRKY39的eDNA基因序列设计引物，克隆水稻日本晴（OryzasativaL．）WRKY类转录因子的eDNA基因并进行原核表达，通过凝胶阻滞实验分析融合蛋白与DNA的结合活性。结果克隆了1个新的WRKY基因OsWRKY39，其编码的蛋白0swRKY39能够与顺式元件W盒特异结合，Os-WRKY39具有转录因子的最基本特征。
    근거수도전록인자WRKY39적eDNA기인서렬설계인물，극륭수도일본청（OryzasativaL．）WRKY류전록인자적eDNA기인병진행원핵표체，통과응효조체실험분석융합단백여DNA적결합활성。결과극륭료1개신적WRKY기인OsWRKY39，기편마적단백0swRKY39능구여순식원건W합특이결합，Os-WRKY39구유전록인자적최기본특정。
    A new rice WRKY gene (OsWRKY39) was cloned from cDNA of young rice leaves employing a PCR strategy and was expressed in E. coli (BL 21 strain). The DNA-binding ac- tivity of the recombinant WRKY39 protein was analyzed using electrophoretic mobility shift assay. It was found that the recombinant WRKY39 protein bound specifically to W-box, which indicated WRKY39 was a transcriptional factor.
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