CAJ | 학술논문

建立了鉴别日本神户牛肉真实性的分子生物学检测技术。通过对来源于10个品种的牛的样品的分析,发现了牛生长激素基因第五外显子的品种间多态性位点,并通过比较分析,确定了日本和牛在这些多态性位点的特异核苷酸组合,建立起了日本神户牛肉的分子生物学识别技术。采取了47份市售牛肉对方法进行了验证,证明该方法在应用于国内市场的神户牛肉的真实性鉴别上,具有很好的特异性,可以用于区分日本神户牛肉和国产牛肉以及国内生产的所谓日本黑牛肉。
건립료감별일본신호우육진실성적분자생물학검측기술。통과대래원우10개품충적우적양품적분석,발현료우생장격소기인제오외현자적품충간다태성위점,병통과비교분석,학정료일본화우재저사다태성위점적특이핵감산조합,건립기료일본신호우육적분자생물학식별기술。채취료47빈시수우육대방법진행료험증,증명해방법재응용우국내시장적신호우육적진실성감별상,구유흔호적특이성,가이용우구분일본신호우육화국산우육이급국내생산적소위일본흑우육。
A method for differentiating Wagyu beef and beef from other breeds was developed.Samples from 10 breeds of bovine were studied,and 3 polymorphism loci were found in the 5th extron of growth hormone gene.There are specific combinations of nucleotides in Wagyu,which provide the basis for the method.47 beef from the market were used to validate the method,which showed a good performance in identifying the real Wagyu beef.