CAJ | 학술논문

对陇东地方牛种及其与秦川牛和南德温牛杂交群体共计113个个体线粒体D-loop区461 bp序列进行多态性分析,结果表明,3个群体该区域A＋T含量（64.2%）明显高于G＋C含量（35.8%）,表现出明显的碱基偏倚;3个群体核苷酸变异率介于0.42%和3.27%之间,陇东牛和南德温牛杂交群体核苷酸变异率最高;核苷酸变异类型以转换为主,转换占核苷酸多态位点的90.94%,其次为颠换,占4.59%,同样表现出明显的碱基替换偏倚;3个群体核苷酸岐异度介于0.008 95和0.029 06之间,遗传距离介于0.000 15和0.008 36之间,陇东地方牛及其与秦川牛的杂交群体核苷酸歧异度和遗传距离最小。
대롱동지방우충급기여진천우화남덕온우잡교군체공계113개개체선립체D-loop구461 bp서렬진행다태성분석,결과표명,3개군체해구역A＋T함량（64.2%）명현고우G＋C함량（35.8%）,표현출명현적감기편의;3개군체핵감산변이솔개우0.42%화3.27%지간,롱동우화남덕온우잡교군체핵감산변이솔최고;핵감산변이류형이전환위주,전환점핵감산다태위점적90.94%,기차위전환,점4.59%,동양표현출명현적감기체환편의;3개군체핵감산기이도개우0.008 95화0.029 06지간,유전거리개우0.000 15화0.008 36지간,롱동지방우급기여진천우적잡교군체핵감산기이도화유전거리최소。
A total of 461 bp of the mitochondrial D-loop region was sequenced and analyzed in 113 individuals from 3 populations,Longdong cattle,Longdong cattle × Qinchuan cattle hybrid population and Longdong cattle × Nandewen cattle hybrid population.The results indicated the content of A +T(64.2%) in D-loop region in all 3 populations was significantly higher than that of G +C(35.8%),showing the base bias;Nucleotide mutation rate in 3 populations was between 0.42% and 3.27%,and the nucleotide mutation rate in Longdong cattle and Longdong cattle × Nandewen cattle hybrid population was the highest;Transition was in the majority with 90.94% in nucleotide polymorphism site,followd by transversion with 4.59%,also showing base substitution bias;the nucleotide divergence in 3 populations was between 0.008 95 and 0.029 06,the genetic distance was between 0.000 15 and 0.008 36,and the nucleotide divergence and net genetic distance beween Longdong cattle and Longdong cattle × Qinchuan cattle hybrid population was the smallest.