CAJ | 학술논문

本试验旨在建立高效液相色谱-蒸发光散射检测器（HPLC-ELSD）测定芪参口服液中黄芪甲苷含量的方法。采用YMC-Pack ODS-A色谱柱（5μm,150 mm×4.6 mm）,以乙腈-水（35∶65）为流动相,流速1.0 mL/min,柱温30℃,飘移管温度65℃,氮气压力30 psi。结果表明,黄芪甲苷对照品在0.60-6.00μg范围内线性关系良好（r=0.999 6）,平均加样回收率为97.88%（n=6）,峰面积的相对标准偏差（RSD）为3.20%。该方法灵敏、准确、重复性好,可作为芪参口服液的质量控制方法。
본시험지재건립고효액상색보-증발광산사검측기（HPLC-ELSD）측정기삼구복액중황기갑감함량적방법。채용YMC-Pack ODS-A색보주（5μm,150 mm×4.6 mm）,이을정-수（35∶65）위류동상,류속1.0 mL/min,주온30℃,표이관온도65℃,담기압력30 psi。결과표명,황기갑감대조품재0.60-6.00μg범위내선성관계량호（r=0.999 6）,평균가양회수솔위97.88%（n=6）,봉면적적상대표준편차（RSD）위3.20%。해방법령민、준학、중복성호,가작위기삼구복액적질량공제방법。
This experiment was conducted to establish a method for determining the content of Astragoloside Ⅳ in Qishen oral solution by HPLC-ELSD.The chromatographic separation was carried out on YMC-Pack ODS-A column(5 μm,150 mm×4.6 mm).The mobile phase composed of methyl cyanide-water(35∶65)was used at the flow rate of 1 mL/min with the column temperature of 30 ℃.The drift tube temperature of the evaporative light-scattering detector was 65 ℃ and N 2 pressure was 30 psi.Astragoloside Ⅳ showed good linearity(r=0.999 6) in the range of 0.60-6.00 μg,and the average recovery was 97.88% with RSD of 3.20%.The method is sensitive,accurate and repeatable,and can be used for the quality control of Qishen oral solution.