CAJ | 학술논문

采集甘肃兰州市区宠物犬体内两株泡状带绦虫,运用分子生物学方法对其线粒体部分基因进行克隆,并使用基因分析软件Larsergene V7.1进行序列分析,结果发现这两株虫体同源性达到99.6%,与国内的泡状带绦虫广东虫株和甘肃虫株同源性分别达到98.7%和98.4%,鉴定为泡状带绦虫;与其他带科绦虫同源性均高于85%,低于88%。其中,与国内牛带绦虫同源性最高,为88%;与国内巨颈带绦虫同源性最低,只有85.1%。推断不同带科绦虫的cox1基因种间差异明显,在带科绦虫的分类上意义较大。
채집감숙란주시구총물견체내량주포상대조충,운용분자생물학방법대기선립체부분기인진행극륭,병사용기인분석연건Larsergene V7.1진행서렬분석,결과발현저량주충체동원성체도99.6%,여국내적포상대조충엄동충주화감숙충주동원성분별체도98.7%화98.4%,감정위포상대조충;여기타대과조충동원성균고우85%,저우88%。기중,여국내우대조충동원성최고,위88%;여국내거경대조충동원성최저,지유85.1%。추단불동대과조충적cox1기인충간차이명현,재대과조충적분류상의의교대。
Partial mitochondrial genome of two strains of Taenia hydatigena in dogs selected in anzhou were amplified by PCR. Their sequence was analyzed with the software Larsergene V7.1 and compared with tile corresponding sequence of Guangdong strain and Gansu strain published in GenBank.It revealed that the two stratus shared 99.6% of sequence similarity, and had a homology of 98.7% and 98.4% with Guangdong strain and Gansu strain, respecfively. Taenia hydatigena in dogs in Lanzhou had a homology of 85%-88% with the other Taeniidae cestodes. It is conch,deal that coxl gene with species-specific may have great significance in the classification of different Taeniidae cestodes.