CAJ | 학술논문

建立一种用于分析正山小种红茶中茶黄素的液相色谱-串联质谱法,利用多重反应监测模式对正山小种红茶中茶黄素进行定性和定量分析.色谱柱为反相C18柱,流动相为0.1%甲酸和乙腈,柱温35℃.分析结果表明：方法检出限为6.6 ng/g,相对标准偏差小于4.08%,回收率为91.2%～102.5%,该方法简便、快速、准确,适用于红茶中茶黄素的分析.
건립일충용우분석정산소충홍다중다황소적액상색보-천련질보법,이용다중반응감측모식대정산소충홍다중다황소진행정성화정량분석.색보주위반상C18주,류동상위0.1%갑산화을정,주온35℃.분석결과표명：방법검출한위6.6 ng/g,상대표준편차소우4.08%,회수솔위91.2%～102.5%,해방법간편、쾌속、준학,괄용우홍다중다황소적분석.
A liquid chromatography coupled with tandem mass spectrometry method for the analysis of theaflavins in black tea is established.The theaflavins in black tea are identified by the HPLC-ESI-MS-MS technique with the multiple-reaction monitoring mode.The separation of theaflavins is performed on sunfire-C18 at 35 ℃,formic acid-0.1% and acetonitrile are used as the mobile phase for elution.The limit of detection is 6.6ng/g,the relative standard deviation is less than 4.08%,the recoveries are 91.2%~102.5%.The results show that the method is simple,rapid and accurate for the determination of theaflavins in black tea.