CAJ | 학술논문

采用离体脑片膜片钳技术,利用GABAA受体阻断剂PTX、NMDA受体阻断剂AP—5,AMPA受体阻断剂CNQX孤立的记录多巴胺能神经元在一系列阶跃电流刺激下产生动作电位的情况,研究中脑腹侧被盖区多巴胺能神经元兴奋性的变化.结果显示,单次注射可卡因后24h,大鼠中脑腹侧被盖区多巴胺能神经元的基强度降低,放电频率增加,细胞的兴奋性增强,其细胞膜的电生理特性发生了改变.
채용리체뇌편막편겸기술,이용GABAA수체조단제PTX、NMDA수체조단제AP—5,AMPA수체조단제CNQX고립적기록다파알능신경원재일계렬계약전류자격하산생동작전위적정황,연구중뇌복측피개구다파알능신경원흥강성적변화.결과현시,단차주사가잡인후24h,대서중뇌복측피개구다파알능신경원적기강도강저,방전빈솔증가,세포적흥강성증강,기세포막적전생리특성발생료개변.
Activation of dopaminergic neurons in ventral tegmental area plays an important role in drug addiction.This experiment used whole-cell patch-clamping in midbrain slices,bathing with antagonist PTX,AP—5,CNQX,giving dopaminergic neurons a series of step current,to explore the intrinsic plasticity in VTA dopaminergic neurons after cocaine exposure.The results showed that rheobase decreased and action potentials frequency increased in neurons from cocaine-treated animals.The results indicated that single cocaine exposure increases the intrinsic excitability in ventral tegmental area dopaminergic neurons.