CAJ | 학술논문

建立了快速、高效的酱油中三氯丙醇的固相萃取净化-气相色谱-串联质谱法（GC—QqQ—MS／MS）测定方法。0．5g酱油，加0．5mL饱和NaCl盐析，超声15min后经弗罗里硅土固相萃取柱净化，无水乙醚洗脱，d，-3-MCPD为内标，七氟丁酰基咪唑衍生化，加饱和NaCl3mL混匀，有机层进GC—QqQ—MS／MS分析。净化时间少于20min。方法回收率为101．36％，线性范围在0．05-5．0mg／ks，线性相关系数R2=O．99997，方法检出限为0．005mg／kg，该方法对酱油中三氯丙醇的测定有良好的效果。
건립료쾌속、고효적장유중삼록병순적고상췌취정화-기상색보-천련질보법（GC—QqQ—MS／MS）측정방법。0．5g장유，가0．5mL포화NaCl염석，초성15min후경불라리규토고상췌취주정화，무수을미세탈，d，-3-MCPD위내표，칠불정선기미서연생화，가포화NaCl3mL혼균，유궤층진GC—QqQ—MS／MS분석。정화시간소우20min。방법회수솔위101．36％，선성범위재0．05-5．0mg／ks，선성상관계수R2=O．99997，방법검출한위0．005mg／kg，해방법대장유중삼록병순적측정유량호적효과。
A fast, effective method for analysis of 3 -MCPD in soy sauces was developed by modified SPE- GC -QqQ- MS/MS method. 0. 5g of soy sauces, which was added 0. 5 mL saturated NaC1, and was extracted by ultrasonic for 15rain, and then was purified by Florisil -SPE. D5 -3 -MCPD and N - Heptafluorobutyrylimidazole was used as the internal standard and derivative reagent respectively The re- covery was 101.36% ,liner ranges were 0.05 -5.0mg/kg,correlation coefficient R2 =0. 99997 ,the limit of detection was O. 005mg/kg. This method meet the requirement of determiantion of 3 - MCPD in soy sauces.