齐鲁师范学院学报
齊魯師範學院學報
제로사범학원학보
JOURNAL OF SHANDONG EDUCATION INSTITUTE
2012年
5期
53-55
,共3页
高效液相色谱%三聚氰胺%乳制品
高效液相色譜%三聚氰胺%乳製品
고효액상색보%삼취청알%유제품
High performance liquid chromatographic%Melamine%Dairy products
优化测定乳制品中三聚氰胺含量的高效液相色谱方法。色谱条件:ODS—SPe18柱,250mm×4.6mm(i.d.),5μm;流动相:A为离子对缓冲液(2.02g庚烷磺酸钠和2.10g柠檬酸,加入约950mL水溶解,调节pH值至3.0后,定容至1L),B为乙腈,配制比例为85%A+15%B;1.0mL/min;柱温:35℃;波长:242nm;选样量:20灿。结果表明:三聚氰胺标准曲线为Y=-5.582+42.55x,相关系数R=0.9997,在1.0-50.0μg/mL范围内线性关系良好.样品三个浓度水平的加标回收率在90.1—105.9%之间,相对标准偏差为2.04-5.26%。
優化測定乳製品中三聚氰胺含量的高效液相色譜方法。色譜條件:ODS—SPe18柱,250mm×4.6mm(i.d.),5μm;流動相:A為離子對緩遲液(2.02g庚烷磺痠鈉和2.10g檸檬痠,加入約950mL水溶解,調節pH值至3.0後,定容至1L),B為乙腈,配製比例為85%A+15%B;1.0mL/min;柱溫:35℃;波長:242nm;選樣量:20燦。結果錶明:三聚氰胺標準麯線為Y=-5.582+42.55x,相關繫數R=0.9997,在1.0-50.0μg/mL範圍內線性關繫良好.樣品三箇濃度水平的加標迴收率在90.1—105.9%之間,相對標準偏差為2.04-5.26%。
우화측정유제품중삼취청알함량적고효액상색보방법。색보조건:ODS—SPe18주,250mm×4.6mm(i.d.),5μm;류동상:A위리자대완충액(2.02g경완광산납화2.10g저몽산,가입약950mL수용해,조절pH치지3.0후,정용지1L),B위을정,배제비례위85%A+15%B;1.0mL/min;주온:35℃;파장:242nm;선양량:20찬。결과표명:삼취청알표준곡선위Y=-5.582+42.55x,상관계수R=0.9997,재1.0-50.0μg/mL범위내선성관계량호.양품삼개농도수평적가표회수솔재90.1—105.9%지간,상대표준편차위2.04-5.26%。
To optimize a quantitative high performance liquid chromatographic method for the simultaneous determination of melamine in dairy products, an HPLC method was set up. Chromatographic condition: The chromat ocolumn was ODS- SP C18 column, 150mm ×4. 6mm (i. d. ) 5urn. The mobile phase consisted of ion-pair buffer (pH =3.0) - acetonitrile (85:15), the flow rate was 1.0 ml/min, the column temperature was 35℃ and the UV detection wavelength was 242 nm. Results: The linearity was obtained over the range of 1. 0 - 50. 0μg/ml ( R = 0.9997), the average recovery was 90.1 - 105.9%, RSD was 2.04 - 5.26%.