CAJ | 학술논문

利用罗非鱼鱼皮提取明胶制备蛋白膜,考察了浸提条件和戊二醛的添加对明胶蛋白膜性质的影响.结果表明,利用罗非鱼鱼皮可以制备成无色透明的明胶蛋白膜.在80℃水浴条件下,伴随着明胶浸提时间的延长,膜的抗拉伸强度（TS）出现先上升后下降的趋势,在浸提时间为1.0 h时达到最大值.而膜的断裂延伸率（EAB）和水蒸气透过率（WVP）则随着浸提时间的增加而下降.然而,浸提时间对膜的透光率没有显著影响.另一方面,当利用质量分数为1%～4%（相对明胶蛋白）的戊二醛对蛋白膜进行改性时,膜的TS、EAB、WVP和溶解性均明显下降,而蛋白膜的色泽变黄,紫外线阻隔能力增强.利用SDS-PAGE分析发现,戊二醛的添加会使明胶分子发生交联反应,且反应更易在高分子成分之间发生.
이용라비어어피제취명효제비단백막,고찰료침제조건화무이철적첨가대명효단백막성질적영향.결과표명,이용라비어어피가이제비성무색투명적명효단백막.재80℃수욕조건하,반수착명효침제시간적연장,막적항랍신강도（TS）출현선상승후하강적추세,재침제시간위1.0 h시체도최대치.이막적단렬연신솔（EAB）화수증기투과솔（WVP）칙수착침제시간적증가이하강.연이,침제시간대막적투광솔몰유현저영향.령일방면,당이용질량분수위1%～4%（상대명효단백）적무이철대단백막진행개성시,막적TS、EAB、WVP화용해성균명현하강,이단백막적색택변황,자외선조격능력증강.이용SDS-PAGE분석발현,무이철적첨가회사명효분자발생교련반응,차반응경역재고분자성분지간발생.
The effect of extraction conditions and glutaraldehyde(GLU)incorporation on the physical and chemical properties of the gelatin films prepared from tilapia skin was investigated.As a result,colorless and transparent edible film could be successfully prepared using tilapia skin gelatin.The tensile strength(TS)of films as prepared from gelatin extracted at 80 ℃ increased and reached a peak after extraction for 1.0 h,while a gradual decrease of TS was observed when the extraction time was increased.The elongation at break(EAB) and water vapor permeability(WVP) of films were enhanced together with the increase of gelatin extraction time at 80 ℃.However,the light transmition of films was not affected by extraction time.On the other hand,when GLU was incorporated in the film-forming solutions at 1%~4%(m/m) of protein,the TS,EAB,WVP and solubility of films all decreased,while the yellowness and barrier properties against UV lights increased.SDS-PAGE analysis indicated that the protein cross-linking reaction mainly involved in high molecular weight polymers was induced by GLU.