CAJ | 학술논문

结合生产实际，采用不同添加量的酵母浸出液、鸡血清、NADH作为半合成培养基的辅料进行Hpg-8株的培养，通过活菌计数及菌液0D600吸光值的测定来比较各试验组的培养效果，并将所获得的培养物适当浓缩后制成油乳剂灭活疫苗免疫60日龄SPF鸡进行免疫原性的测定。最后，综合考虑各组培养物的抗原浓度、单位生产成本及免疫原性，确定使用在半合成培养基中添加5％鸡血清、10％酵母浸出液和0．0015％NADH的培养方案进行Hpg-8株的培养，采用该培养条件所获得的菌液的活菌数可达2．04×10^9CFU／mL，其免疫原性也符合中华人民共和国兽药典对Hpg-8株制苗抗原的要求。
결합생산실제，채용불동첨가량적효모침출액、계혈청、NADH작위반합성배양기적보료진행Hpg-8주적배양，통과활균계수급균액0D600흡광치적측정래비교각시험조적배양효과，병장소획득적배양물괄당농축후제성유유제멸활역묘면역60일령SPF계진행면역원성적측정。최후，종합고필각조배양물적항원농도、단위생산성본급면역원성，학정사용재반합성배양기중첨가5％계혈청、10％효모침출액화0．0015％NADH적배양방안진행Hpg-8주적배양，채용해배양조건소획득적균액적활균수가체2．04×10^9CFU／mL，기면역원성야부합중화인민공화국수약전대Hpg-8주제묘항원적요구。
In this present study different amount of yeast extract, chicken serum and NADH were used as the additives for the semisynthesis culture medium to prepare strain Hpg-8. The cultivation result of each experiment group was evaluated by comparing the viable count and the OD600 value of the culture, and the immunogenicity of the culture were evaluated by the challenge protection test. Considering on the antigen concentration, the unit production costs, and the immunogenicity of each group, the optimal amount of the yeast extract, chicken serum and NADH was determined to be 10%, 5% or 0.0015% respectively. Using this cultivating condition, the viable count could reach 2.04 billion and the immunogenicity of the culture conformed with the requirements of the Veterinary Pharmacopoeia of the People's Republic of China.