广东畜牧兽医科技
廣東畜牧獸醫科技
엄동축목수의과기
GUANDONG JOURNAL OF ANIMAL AND VETERINARY SCIENCE
2012年
5期
29-33
,共5页
陶光灿%孙震%杨昌松%冯静%韩京朋
陶光燦%孫震%楊昌鬆%馮靜%韓京朋
도광찬%손진%양창송%풍정%한경붕
喹诺酮类药物%残留%间接竞争酶联免疫法%液相色谱-串联质谱法
喹諾酮類藥物%殘留%間接競爭酶聯免疫法%液相色譜-串聯質譜法
규낙동류약물%잔류%간접경쟁매련면역법%액상색보-천련질보법
Quinolones%Residue%ELISA%LC-MS/MS
分别采用间接竞争酶联免疫法（ELISA）与液相色谱-串联质谱（LC—MS／MS）法对罗非鱼肉中喹诺酮类药物残留量进行检测，对得出的检测结果进行比较分析。结果显示：ELISA方法样本添加回收率在73．4％～89．5％之间，变异系数在5．6％～14．3％之间，检测时间为45min，适合于罗非鱼肉中喹诺酮类药物残留的快速筛选；LC-Ms／Ms方法，样本添加回收率为77．1％～85．7％，变异系数在5．5％～11．2％之间，检测结果准确，变异系数较小，但检测费用较高，适用于喹诺酮类药物残留的确证。
分彆採用間接競爭酶聯免疫法（ELISA）與液相色譜-串聯質譜（LC—MS／MS）法對囉非魚肉中喹諾酮類藥物殘留量進行檢測，對得齣的檢測結果進行比較分析。結果顯示：ELISA方法樣本添加迴收率在73．4％～89．5％之間，變異繫數在5．6％～14．3％之間，檢測時間為45min，適閤于囉非魚肉中喹諾酮類藥物殘留的快速篩選；LC-Ms／Ms方法，樣本添加迴收率為77．1％～85．7％，變異繫數在5．5％～11．2％之間，檢測結果準確，變異繫數較小，但檢測費用較高，適用于喹諾酮類藥物殘留的確證。
분별채용간접경쟁매련면역법（ELISA）여액상색보-천련질보（LC—MS／MS）법대라비어육중규낙동류약물잔류량진행검측，대득출적검측결과진행비교분석。결과현시：ELISA방법양본첨가회수솔재73．4％～89．5％지간，변이계수재5．6％～14．3％지간，검측시간위45min，괄합우라비어육중규낙동류약물잔류적쾌속사선；LC-Ms／Ms방법，양본첨가회수솔위77．1％～85．7％，변이계수재5．5％～11．2％지간，검측결과준학，변이계수교소，단검측비용교고，괄용우규낙동류약물잔류적학증。
The quinolones residue in tilapia was detected by the methods of enzyme-linked immunosorbent assay(ELISA) and liquid chromatography-tandem mass spectrometry(LC-MS/MS). The results showed that in the ELISA test the recovery rate was from 73.4% to 89.5%, the coefficient of variation(CV)% was from 5.6% to 14.3%, the detection time was within 45 minutes and in the LC-MS/MS test the recovery rate was from 77.1% to 85.7%, the CV% was from 5.5% to 11.2%. The results of LC-MS/MS was more accurate and the CV% of LC-MS/MS was more small than the results of ELISA, but the test of LC-MS/MS was more expensive. So the ELISA test was suitable for the rapid screening of the quinolones residue in tilapia and the LC-MS/MS was only suitable for confirmation.
