国际医药卫生导报
國際醫藥衛生導報
국제의약위생도보
INTERNATIONAL MEDICINE & HEALTH GUIDANCE NEWS
2013年
18期
2817-2820
,共4页
陈志山%洪黎清%杨昌毅%傅少龙
陳誌山%洪黎清%楊昌毅%傅少龍
진지산%홍려청%양창의%부소룡
直肠癌%细胞株%Yes相关蛋白%mRNA
直腸癌%細胞株%Yes相關蛋白%mRNA
직장암%세포주%Yes상관단백%mRNA
Colorectal cancer%Cell lines%Yes-associated protein%mRNA
目的 研究YAP在直肠癌Colo320细胞株和直肠癌淋巴结阳性及阴性中的表达情况,探讨其临床意义.方法 应用S-P免疫组织化学和RT-PCR检测Colo320细胞株中Yes相关蛋白及mRNA的表达;应用RT-PCR检测10例直肠癌转移淋巴结和阴性淋巴结中YAPmRNA的表达.结果 Colo320细胞株中YAP蛋白及mRNA的表达呈阳性;直肠癌转移淋巴结中YAPmRNA的表达水平高于淋巴结阴性(0.46±0.12vs.0.15±0.08,P＜0.05).结论 YAP在直肠癌Colo320细胞株呈阳性表达,YAP与直肠癌的淋巴结转移有一定的关系.
目的 研究YAP在直腸癌Colo320細胞株和直腸癌淋巴結暘性及陰性中的錶達情況,探討其臨床意義.方法 應用S-P免疫組織化學和RT-PCR檢測Colo320細胞株中Yes相關蛋白及mRNA的錶達;應用RT-PCR檢測10例直腸癌轉移淋巴結和陰性淋巴結中YAPmRNA的錶達.結果 Colo320細胞株中YAP蛋白及mRNA的錶達呈暘性;直腸癌轉移淋巴結中YAPmRNA的錶達水平高于淋巴結陰性(0.46±0.12vs.0.15±0.08,P＜0.05).結論 YAP在直腸癌Colo320細胞株呈暘性錶達,YAP與直腸癌的淋巴結轉移有一定的關繫.
목적 연구YAP재직장암Colo320세포주화직장암림파결양성급음성중적표체정황,탐토기림상의의.방법 응용S-P면역조직화학화RT-PCR검측Colo320세포주중Yes상관단백급mRNA적표체;응용RT-PCR검측10례직장암전이림파결화음성림파결중YAPmRNA적표체.결과 Colo320세포주중YAP단백급mRNA적표체정양성;직장암전이림파결중YAPmRNA적표체수평고우림파결음성(0.46±0.12vs.0.15±0.08,P＜0.05).결론 YAP재직장암Colo320세포주정양성표체,YAP여직장암적림파결전이유일정적관계.
Objective To study the expression of YAP in colorectal cancer Colo320 cell lines and cancer lymph nodes-positive and negative.Methods S-P immunohistochemistry and reverse transcriptase polymerase chain reaction (RT-PCR) were used to detect protein expression and mRNA.RT-PCR analysis was used to detect the expression of YAP mRNA in 10 patients with rectal cancer metastatic lymph nodes and negative lymph nodes.Results YAP protein and mRNA showed expression of positive Colo320 cell lines.Rectal cancer metastasis lymph nodes YAP mRNA' s expression level was higher than that of the node-negative (0.46 ± 0.12vs.0.15 ± 0.08,P ＜0.05).Conclusion YAP presented positive expression in rectal cancer Colo320 cell lines.YAP high expression is related to the rectal cancer lymph node metastasis.
