宜春学院学报
宜春學院學報
의춘학원학보
JOURNAL OF YICHUN UNIVERSITY
2012年
8期
102-104
,共3页
王爱珠%唐敏贤%林明道%钟秀英
王愛珠%唐敏賢%林明道%鐘秀英
왕애주%당민현%림명도%종수영
溴氰菊酯%血红素加氧酶%ATPase%活性
溴氰菊酯%血紅素加氧酶%ATPase%活性
추청국지%혈홍소가양매%ATPase%활성
Deltamethrin%Heme Oxygenase Activity%ATPase
目的:研究溴氰菊酯(DM)对SD大鼠小脑皮质组织中血红素加氧酶(HO)的活性和ATPase活性的影响。方法:(1)成年雄性SD大鼠共40只,其中20只用于血红素加氧酶(HO)的活性的测定,另20只用于ATPase活力测定,均随机分为对照组和DM染毒实验组,实验组中大鼠72小时内给溴氰菊酯(1/20LD50)三次,对照组为相同剂量的玉米油,分别检测小脑皮质组织HO活性和ATPase活性。(2)成年雄性SD大鼠30只,同样方法取出小脑皮质,制成切片,随机分为对照组(相同剂量的玉米油)和5个不同浓度的DM染毒试验组(2×10-7mmol/L、2×10-6mmol/L、2×10-5mmol/L、2×10-4mmol/L、2×10-3mmol/L),测定ATPase活性。结果:整体染毒小脑皮层组织HO活性为对照组的240.39%(p﹤0.05),Na+,K+-ATPase活性为对照组的131.47%(p﹤0.05),Ca2+-ATPase活性增加没有统计学意义,Mg2+-ATPase有显著的抑制作用(P〈0.05),为对照组的79.91%。体外试验发现只有达到10-3mmol/L数量级的DM对温育小脑皮质切片组织中的Mg2+-ATPase活性才有显著的抑制作用(P〈0.05);且无论何种浓度均未发现上述体内染毒时对Na+,K+-ATPase活性的增加作用。结论:DM可增加大鼠小脑皮质的HO活性,体内体外染毒DM对三种ATPase活性的影响并不一致。
目的:研究溴氰菊酯(DM)對SD大鼠小腦皮質組織中血紅素加氧酶(HO)的活性和ATPase活性的影響。方法:(1)成年雄性SD大鼠共40隻,其中20隻用于血紅素加氧酶(HO)的活性的測定,另20隻用于ATPase活力測定,均隨機分為對照組和DM染毒實驗組,實驗組中大鼠72小時內給溴氰菊酯(1/20LD50)三次,對照組為相同劑量的玉米油,分彆檢測小腦皮質組織HO活性和ATPase活性。(2)成年雄性SD大鼠30隻,同樣方法取齣小腦皮質,製成切片,隨機分為對照組(相同劑量的玉米油)和5箇不同濃度的DM染毒試驗組(2×10-7mmol/L、2×10-6mmol/L、2×10-5mmol/L、2×10-4mmol/L、2×10-3mmol/L),測定ATPase活性。結果:整體染毒小腦皮層組織HO活性為對照組的240.39%(p﹤0.05),Na+,K+-ATPase活性為對照組的131.47%(p﹤0.05),Ca2+-ATPase活性增加沒有統計學意義,Mg2+-ATPase有顯著的抑製作用(P〈0.05),為對照組的79.91%。體外試驗髮現隻有達到10-3mmol/L數量級的DM對溫育小腦皮質切片組織中的Mg2+-ATPase活性纔有顯著的抑製作用(P〈0.05);且無論何種濃度均未髮現上述體內染毒時對Na+,K+-ATPase活性的增加作用。結論:DM可增加大鼠小腦皮質的HO活性,體內體外染毒DM對三種ATPase活性的影響併不一緻。
목적:연구추청국지(DM)대SD대서소뇌피질조직중혈홍소가양매(HO)적활성화ATPase활성적영향。방법:(1)성년웅성SD대서공40지,기중20지용우혈홍소가양매(HO)적활성적측정,령20지용우ATPase활력측정,균수궤분위대조조화DM염독실험조,실험조중대서72소시내급추청국지(1/20LD50)삼차,대조조위상동제량적옥미유,분별검측소뇌피질조직HO활성화ATPase활성。(2)성년웅성SD대서30지,동양방법취출소뇌피질,제성절편,수궤분위대조조(상동제량적옥미유)화5개불동농도적DM염독시험조(2×10-7mmol/L、2×10-6mmol/L、2×10-5mmol/L、2×10-4mmol/L、2×10-3mmol/L),측정ATPase활성。결과:정체염독소뇌피층조직HO활성위대조조적240.39%(p﹤0.05),Na+,K+-ATPase활성위대조조적131.47%(p﹤0.05),Ca2+-ATPase활성증가몰유통계학의의,Mg2+-ATPase유현저적억제작용(P〈0.05),위대조조적79.91%。체외시험발현지유체도10-3mmol/L수량급적DM대온육소뇌피질절편조직중적Mg2+-ATPase활성재유현저적억제작용(P〈0.05);차무론하충농도균미발현상술체내염독시대Na+,K+-ATPase활성적증가작용。결론:DM가증가대서소뇌피질적HO활성,체내체외염독DM대삼충ATPase활성적영향병불일치。
Objective:To study the effects of deltamethrin(DM) on heme oxygenase(HO) activity and ATPase activity in SD rat’s cerebellar cortex tissue.Methods:(1) There were 40adult male SD rats,of which 20 was only used for heme oxygenase(HO) activity determination,and the other 20 were used only for ATPase activity determination.They were randomly divided into control group and DM experimental group.The rats in experimental group were exposed to deltamethrin(1/20 LD50 three times within 72 hours,while control group were given corn oil with the same dose.They both were used to detect HO activity and ATPase activity in the cerebellar cortex tissue.(2) Cerebellar cortexes were removed from 30 adult male SD rats in the same way and were cut into slices.They were randomly divided into control group(corn oil with the same dose) and 5 experimental groups with different concentrations,which were exposed to DM(2×10-7mmol/L,2×10-6mmol/L,2×10-5mmol/L,2×10-4mmol/L,2×10-3mmol/L).ATPase activity was determined.Results:The HO activity in cerebellar cortical tissue after overall exposure was 240.39% in comparison with the control group(P〈0.05).Na+,the activity of K+-ATPase was 131.47% in comparison with the control group(P〈0.05).Increased Ca2+-ATPase activity was not statistically significant.Mg2+-ATPase was significantly inhibited(P〈0.05),as compared to a control group of 79.91%.In vitro tests have found only when DM reached 10-3mmol/L orders of magnitude can it inhibit Mg2+-ATPase in cerebellar cortex slices significantly(P〈0.05);and whatever the concentration was,increased role in Na+ and K+-ATPase activity was not found in the in vivo exposure.Conclusion:DM can increase the HO activity in rat cerebellar cortex.Effects of DM exposed in vitro and in vivo on three kinds of ATPase activity are not consistent.