中国食物与营养
中國食物與營養
중국식물여영양
Food and Nutrition in China
2012年
9期
70-72
,共3页
ICA%大鼠%卵巢%RLF
ICA%大鼠%卵巢%RLF
ICA%대서%란소%RLF
ICA%rat%ovaries%RLF
目的：研究淫羊藿苷（ICA）对大鼠卵巢功能与松弛素样因子（relaxin-like factor,RLF）mRNA表达的影响,探讨其对大鼠卵巢功能的调节作用机制。方法：大鼠按ICA低、中、高剂量隔日给药,连续30d;雌二醇按雌二醇放射免疫试剂盒步骤测定;RT-PCR法测定RLF mRNA的表达量。结果：ICA能够增加大鼠卵巢重量,促进卵泡发育,提高雌激素分泌量,但不扰乱动情周期;ICA中剂量组和高剂量组均使RLF mRNA表达增加（P〈0.05）。结论：ICA具有促进雌性生殖的作用,可通过上调RLF mRNA表达来实现。
目的：研究淫羊藿苷（ICA）對大鼠卵巢功能與鬆弛素樣因子（relaxin-like factor,RLF）mRNA錶達的影響,探討其對大鼠卵巢功能的調節作用機製。方法：大鼠按ICA低、中、高劑量隔日給藥,連續30d;雌二醇按雌二醇放射免疫試劑盒步驟測定;RT-PCR法測定RLF mRNA的錶達量。結果：ICA能夠增加大鼠卵巢重量,促進卵泡髮育,提高雌激素分泌量,但不擾亂動情週期;ICA中劑量組和高劑量組均使RLF mRNA錶達增加（P〈0.05）。結論：ICA具有促進雌性生殖的作用,可通過上調RLF mRNA錶達來實現。
목적：연구음양곽감（ICA）대대서란소공능여송이소양인자（relaxin-like factor,RLF）mRNA표체적영향,탐토기대대서란소공능적조절작용궤제。방법：대서안ICA저、중、고제량격일급약,련속30d;자이순안자이순방사면역시제합보취측정;RT-PCR법측정RLF mRNA적표체량。결과：ICA능구증가대서란소중량,촉진란포발육,제고자격소분비량,단불우란동정주기;ICA중제량조화고제량조균사RLF mRNA표체증가（P〈0.05）。결론：ICA구유촉진자성생식적작용,가통과상조RLF mRNA표체래실현。
【Objective】 To study the effects of icariin on function and RLF mRNA expression in the rat ovaries and to discuss the regulation mechanism of icariin on function of the rat ovary.【Method】 Rats were fed with various of ICA for 30d,the level of estradiol were detected by immunohistochemistry,and the expression of RLF mRNA was detected by RT-PCR.【Result】 ICA could increase the rat weight,promote rat ovaries growth,and improve estradiol secretion,but estrus cycle was right.【Conclusion】 ICA could improve female production function by up regulation.
