国际医药卫生导报
國際醫藥衛生導報
국제의약위생도보
INTERNATIONAL MEDICINE & HEALTH GUIDANCE NEWS
2013年
18期
2820-2823
,共4页
杨羚%戴碧云%罗毓婷%陈定强
楊羚%戴碧雲%囉毓婷%陳定彊
양령%대벽운%라육정%진정강
大肠埃希菌%肺炎克雷伯菌%质粒介导的喹诺酮耐药基因%多重耐药
大腸埃希菌%肺炎剋雷伯菌%質粒介導的喹諾酮耐藥基因%多重耐藥
대장애희균%폐염극뢰백균%질립개도적규낙동내약기인%다중내약
Escherichia coli%Klebsiella pneumoniae%Plasmid-mediated quinolone resistant genes%Multi-drug resistance
目的 分析广州医学院第一附属医院临床分离的60株多重耐药大肠埃希菌和肺炎克雷伯菌质粒介导的喹诺酮耐药基因存在状况.方法 采用VITEK-2全自动细菌鉴定仪检测大肠埃希菌和肺炎克雷伯菌对抗生素的药物敏感性,选择多重耐药的大肠埃希菌和肺炎克雷伯菌作为研究对象,用多重PCR法进行质粒介导的喹诺酮耐药基因的检测和分析.结果 检测出耐药基因qnrA型2株,qnrB型耐药基因15株,aac(6’)-Ib基因9株,其中aac(6’)-Ib-cr型耐药基因5株.其中有1株肺炎克雷伯菌检测到同时含有qnrA和aac(6’)-Ib-cr基因;还有3株肺炎克雷伯菌检测到同时含有qnrB和aac(6’)-Ib-cr基因.未检测到qnrC、qnrD、qnrS、qepA型的耐药基因.结论 临床分离的多重耐药大肠埃希菌和肺炎克雷伯菌含有多种质粒介导的喹诺酮耐药基因,应引起临床的重视.
目的 分析廣州醫學院第一附屬醫院臨床分離的60株多重耐藥大腸埃希菌和肺炎剋雷伯菌質粒介導的喹諾酮耐藥基因存在狀況.方法 採用VITEK-2全自動細菌鑒定儀檢測大腸埃希菌和肺炎剋雷伯菌對抗生素的藥物敏感性,選擇多重耐藥的大腸埃希菌和肺炎剋雷伯菌作為研究對象,用多重PCR法進行質粒介導的喹諾酮耐藥基因的檢測和分析.結果 檢測齣耐藥基因qnrA型2株,qnrB型耐藥基因15株,aac(6’)-Ib基因9株,其中aac(6’)-Ib-cr型耐藥基因5株.其中有1株肺炎剋雷伯菌檢測到同時含有qnrA和aac(6’)-Ib-cr基因;還有3株肺炎剋雷伯菌檢測到同時含有qnrB和aac(6’)-Ib-cr基因.未檢測到qnrC、qnrD、qnrS、qepA型的耐藥基因.結論 臨床分離的多重耐藥大腸埃希菌和肺炎剋雷伯菌含有多種質粒介導的喹諾酮耐藥基因,應引起臨床的重視.
목적 분석엄주의학원제일부속의원림상분리적60주다중내약대장애희균화폐염극뢰백균질립개도적규낙동내약기인존재상황.방법 채용VITEK-2전자동세균감정의검측대장애희균화폐염극뢰백균대항생소적약물민감성,선택다중내약적대장애희균화폐염극뢰백균작위연구대상,용다중PCR법진행질립개도적규낙동내약기인적검측화분석.결과 검측출내약기인qnrA형2주,qnrB형내약기인15주,aac(6’)-Ib기인9주,기중aac(6’)-Ib-cr형내약기인5주.기중유1주폐염극뢰백균검측도동시함유qnrA화aac(6’)-Ib-cr기인;환유3주폐염극뢰백균검측도동시함유qnrB화aac(6’)-Ib-cr기인.미검측도qnrC、qnrD、qnrS、qepA형적내약기인.결론 림상분리적다중내약대장애희균화폐염극뢰백균함유다충질립개도적규낙동내약기인,응인기림상적중시.
Objective To analyze the presence of plasmid-mediated quinolone resistant genes 60 strains of multi-drug resistant Escherichia coli and Klebsiella pneumoniae isolated from the First Affiliated Hospital of Guangzhou Medical College.Methods Using VITEK-2 automatic identification system,antibiotic susceptibility of E.coli and K.pneumoniae were tested.Multi-drug resistant E.coii and K.pneumoniae were selected for research.The presence of plasmid-mediated quinolone resistant genes were detected by multiplex PCR.Results qnrA genes were detected in 2 strains,qnrB were detected in 15 strains and aac (6')-Ib gene were detected in 9 strains,with aac (6')-Ib-cr in 5 strains.One strain of K.pneumoniae c aorried both qnrA and aac (6')-Ib-cr genes and 3 strains of K.pneumoniae carried both qnrB and aac (6')-Ib-cr genes.qnrC,qnrD,qnrS,qepA genes were not detected.Conclusion Clinical isolates of multi-drug resistant E.coli and K.pneumoniae carried multiple plasmid mediated quinolone resistant genes,which should be drew more attention in clinic.
