浙江中西医结合杂志
浙江中西醫結閤雜誌
절강중서의결합잡지
ZHEJIANG JOURNAL OF INTEGRATED TRADITIONAL CHINESE AND WESTERN MEDICINE
2014年
4期
287-289
,共3页
大鼠%哮喘%芍药甘草汤%信号传导和转录激活因子6%白介素-13
大鼠%哮喘%芍藥甘草湯%信號傳導和轉錄激活因子6%白介素-13
대서%효천%작약감초탕%신호전도화전록격활인자6%백개소-13
asthma%Shaoyaogancaotang%signal transducer and activator of transcription 6%interleukin-13
目的：观察芍药甘草汤对哮喘大鼠信号传导和转录激活因子6（STAT 6）与白介素-13（IL-13）水平的干预作用，探讨其防治支气管哮喘的作用机制。方法60只SD雄性大鼠随机分为正常对照组（NULL组）、模型组（OVA组）、地塞米松组（DXM组）及芍药甘草汤高（SG10组）、中（SG5组）、低（SG2.5组）剂量组，每组10只。除正常对照组外均行支气管哮喘造模，相应药物灌胃。检测各组大鼠支气管肺泡灌洗液中白细胞和嗜酸性粒细胞计数，免疫组化法测肺组织中STAT 6蛋白表达，ELISA法检测血浆中IL-13含量。结果与NULL组比较，OVA组哮喘大鼠肺泡灌洗液（BALF）中白细胞总数、嗜酸性粒细胞计数及气道上皮STAT 6表达和血浆IL-13水平均明显升高（P<0.01）。DXM组及各剂量芍药甘草汤组BALF中白细胞总数和嗜酸性粒细胞计数、气道上皮STAT 6表达和血浆IL-13水平较OVA组降低（P<0.01），但仍高于NULL组（P<0.01）。结论芍药甘草汤防治大鼠支气管哮喘的作用机制可能与抑制哮喘大鼠肺组织STAT 6表达，降低血浆IL-13水平，降低哮喘大鼠BALF中白细胞总数及嗜酸性粒细胞计数有关。
目的：觀察芍藥甘草湯對哮喘大鼠信號傳導和轉錄激活因子6（STAT 6）與白介素-13（IL-13）水平的榦預作用，探討其防治支氣管哮喘的作用機製。方法60隻SD雄性大鼠隨機分為正常對照組（NULL組）、模型組（OVA組）、地塞米鬆組（DXM組）及芍藥甘草湯高（SG10組）、中（SG5組）、低（SG2.5組）劑量組，每組10隻。除正常對照組外均行支氣管哮喘造模，相應藥物灌胃。檢測各組大鼠支氣管肺泡灌洗液中白細胞和嗜痠性粒細胞計數，免疫組化法測肺組織中STAT 6蛋白錶達，ELISA法檢測血漿中IL-13含量。結果與NULL組比較，OVA組哮喘大鼠肺泡灌洗液（BALF）中白細胞總數、嗜痠性粒細胞計數及氣道上皮STAT 6錶達和血漿IL-13水平均明顯升高（P<0.01）。DXM組及各劑量芍藥甘草湯組BALF中白細胞總數和嗜痠性粒細胞計數、氣道上皮STAT 6錶達和血漿IL-13水平較OVA組降低（P<0.01），但仍高于NULL組（P<0.01）。結論芍藥甘草湯防治大鼠支氣管哮喘的作用機製可能與抑製哮喘大鼠肺組織STAT 6錶達，降低血漿IL-13水平，降低哮喘大鼠BALF中白細胞總數及嗜痠性粒細胞計數有關。
목적：관찰작약감초탕대효천대서신호전도화전록격활인자6（STAT 6）여백개소-13（IL-13）수평적간예작용，탐토기방치지기관효천적작용궤제。방법60지SD웅성대서수궤분위정상대조조（NULL조）、모형조（OVA조）、지새미송조（DXM조）급작약감초탕고（SG10조）、중（SG5조）、저（SG2.5조）제량조，매조10지。제정상대조조외균행지기관효천조모，상응약물관위。검측각조대서지기관폐포관세액중백세포화기산성립세포계수，면역조화법측폐조직중STAT 6단백표체，ELISA법검측혈장중IL-13함량。결과여NULL조비교，OVA조효천대서폐포관세액（BALF）중백세포총수、기산성립세포계수급기도상피STAT 6표체화혈장IL-13수평균명현승고（P<0.01）。DXM조급각제량작약감초탕조BALF중백세포총수화기산성립세포계수、기도상피STAT 6표체화혈장IL-13수평교OVA조강저（P<0.01），단잉고우NULL조（P<0.01）。결론작약감초탕방치대서지기관효천적작용궤제가능여억제효천대서폐조직STAT 6표체，강저혈장IL-13수평，강저효천대서BALF중백세포총수급기산성립세포계수유관。
Objective To observe the effect of Shaoyaogancaotang(SG) on signal transducer and activator of tran-scription 6(STAT6) and interleukin-13 in asthmatic rat, and to explore the possible mechanism of SG to asthma. Methods Sixty SD rats were randomly divided into 6 groups: normal group(group NULL), asthmatic group(group OVA), dexamethasone group(group DXM), group SG10, group SG5, and group SG2.5. Before nebulization, group NULL and OVA was given saline of 10ml/kg intragastrically, group DXM was given DXM of 1 mg/kg, and groups SG were given SG of 10, 5, and 2.5 g/kg, respectively. The numbers of total leukocytes and eosinophils in bron-choalveolar lavage fluid(BALF) were measured by light microscope. The protein expression of STAT6 in airway ep-ithelium was shown by immunohistochemistry. The serum IL-13 were detected by ELISA. Results After sensitized and challenged, the numbers of total leukocytes and eosinophils in BALF of OVA-treated rats significantly in creased compared with group NULL(P<0.01). After treatment with different dosages of SG and dexamethasone, the numbers of total leukocytes and eosinophils in BALF, the STAT6 protein expression, and serum IL-13 were signifi-cantly lower than those in group OVA(P<0.01), but higher than those in group NULL(P<0.01). Conclusion Shaoyao-gancaotang can significantly inhibit the protein expression of STAT6 in pulmonary tissue, decrease the IL-SG can inhibit the protein expression of STAT6 in pulmonary tissue, and decrease the serum IL-13 and the numbers of to-tal leukocytes and eosinophils in BALF. SG might be of potential value in protection and treatment of asthma.
