CAJ | 학술논문

基因敲除技术是以DNA同源重组技术和胚胎干细胞技术为基础，对特定基因位点进行修饰的实验方法，是揭示基因功能最直接的手段之一。小鼠和人的基因相似性达95％。小鼠培育简单、价格便宜、繁殖能力强、生命周期短，适用于疾病发生发展的全过程观察。利用基因操作技术改造小鼠基因便可产生模拟人类基因变异导致的表型效应，可为人类疾病模型的复制及疾病防治研究提供一种有效的实验手段。随着基因操作技术的不断开发与改进，基因工程化小鼠模型在胃癌研究中将扮演更加重要的角色。本文系统介绍了基因工程小鼠模型在胃肠道肿瘤研究中的应用。
기인고제기술시이DNA동원중조기술화배태간세포기술위기출，대특정기인위점진행수식적실험방법，시게시기인공능최직접적수단지일。소서화인적기인상사성체95％。소서배육간단、개격편의、번식능력강、생명주기단，괄용우질병발생발전적전과정관찰。이용기인조작기술개조소서기인편가산생모의인류기인변이도치적표형효응，가위인류질병모형적복제급질병방치연구제공일충유효적실험수단。수착기인조작기술적불단개발여개진，기인공정화소서모형재위암연구중장분연경가중요적각색。본문계통개소료기인공정소서모형재위장도종류연구중적응용。
The gene knockout is based on the DNA homologous recombination and embryonic stem cell technology. It is an experimental method for modification of specific gene loci, which is one of the most direct means to clarifying gene functions. The similarity of genomes between mouse and human is as high as up to 95%. The life cycle of mice is relatively short, and easy to breed with stronger reproductive capacity. Therefore, the knockout mice are suitable for observing the whole process of disease pathogenesis. With genetic manipulation technology, researchers can produce the simulations of phenotypic effect by human genetic variation. This article focuses on genetically engineered mice models and their application in gastrointestinal cancer.