CAJ | 학술논문

目的：观察降脂消斑片对VEGF介导的AS斑块中炎症反应的影响。方法：运用高脂高胆固醇饲料喂养12周造成日本大耳白兔动脉粥样硬化模型，用降脂消斑片灌胃给药治疗4周，采用双抗体夹心酶联免疫吸附法检测血清中 ICAM -1、MMP -9的含量；采用免疫组化法分析 VEGF、ICAM -1、MMP-9在主动脉粥样斑块中的表达量。结果：与模型组比较，降脂消斑片能明显降低血清中ICAM-1、MMP-9的含量，差异均具有统计学意义（ P＜0．01）；同时也能明显降低VEGF、ICAM-1、MMP-9在主动脉粥样斑块中的面密度，差异均具有统计学意义（ P＜0．01）。结论：降脂消斑片抑制AS斑块内VEGF的表达，进而降低AS斑块中ICAM-1、MMP-9的表达，抑制斑块内炎症反应，从而达到稳定AS斑块作用，延缓AS的进展，这可能是其抗AS作用的机制之一。
목적：관찰강지소반편대VEGF개도적AS반괴중염증반응적영향。방법：운용고지고담고순사료위양12주조성일본대이백토동맥죽양경화모형，용강지소반편관위급약치료4주，채용쌍항체협심매련면역흡부법검측혈청중 ICAM -1、MMP -9적함량；채용면역조화법분석 VEGF、ICAM -1、MMP-9재주동맥죽양반괴중적표체량。결과：여모형조비교，강지소반편능명현강저혈청중ICAM-1、MMP-9적함량，차이균구유통계학의의（ P＜0．01）；동시야능명현강저VEGF、ICAM-1、MMP-9재주동맥죽양반괴중적면밀도，차이균구유통계학의의（ P＜0．01）。결론：강지소반편억제AS반괴내VEGF적표체，진이강저AS반괴중ICAM-1、MMP-9적표체，억제반괴내염증반응，종이체도은정AS반괴작용，연완AS적진전，저가능시기항AS작용적궤제지일。
Objective:To observe the effects of Jiangzhi Xiaoban pills on inflammatory response in atherosclero -sis.Methods:Use the high cholesterol diet to induce the rabbit models for 12 weeks, then fill the Jiangzhi Xi-aoban pills to the stomach for four weeks , finally detect the serum levels of ICAM -1 ,MMP-9 in the rabbits by double antibody sandwich enzyme -linked immunosorbent method and the expression levels of VEGF , ICAM-1,MMP-9 in the atherosclerotic plaques by immunohistochemical staining .Results:Compared with the model group,the Jiangzhi Xiaoban pills could decrease significantly the serum levels of ICAM -1,MMP-9,and the difference was statistically significant (P<0.01);meanwhile,the Jiangzhi Xiaoban pills could de-crease significantly the surface density of VEGF ,ICAM-1 ,MMP-9 in atherosclerotic plaques ,and the differ-ence was statistically significant ( P<0 .01 ) .Conclusion:The Jiangzhi Xiaoban pills inhibit the expression lev-els of VEGF,then they also decrease the level of MMP -9 and ICAM-1 in the atherosclerotic plaques ,and block the inflammatory response .ln these ways , Jiangzhi Xiaoban pills could stabilize the atherosclerotic plaques and delay the progress of atherosclemsis , which may be the mechanisms to resist the atherosclerotic plaques.