CAJ | 학술논문

目的：观察不同工艺提取的麻疯树籽油杀灭血吸虫尾蚴的效果，以筛选制备麻疯树籽油的最佳工艺及配方。方法采用6种不同工艺提取的麻疯树籽油，进行杀灭血吸虫尾蚴即时接触试验。采用麻疯树籽油及其与不同添加剂制成的制剂，进行昆明小鼠血吸虫尾蚴即时接触感染试验，观察其抗血吸虫感染的减虫率。结果95%乙醇回流、料液比1∶8、2 h提取工艺出油率达30.7%。即时接触试验6种麻疯树籽油30 min内将尾蚴全部杀灭。即时接触感染试验麻风树籽油原液减虫率达70.97%，添加苯甲酸苄酯减虫率为58.87%，单一添加月桂氮酮减虫率达77.42%，添加苯甲酸苄酯、邻苯二甲酸二丁酯、月桂氮酮减虫率达100%。结论95%乙醇回流、料液比1∶8、2 h提取工艺为麻疯树籽油最佳提取工艺。麻疯树籽油有较好的血吸虫尾蚴杀灭作用。
목적：관찰불동공예제취적마풍수자유살멸혈흡충미유적효과，이사선제비마풍수자유적최가공예급배방。방법채용6충불동공예제취적마풍수자유，진행살멸혈흡충미유즉시접촉시험。채용마풍수자유급기여불동첨가제제성적제제，진행곤명소서혈흡충미유즉시접촉감염시험，관찰기항혈흡충감염적감충솔。결과95%을순회류、료액비1∶8、2 h제취공예출유솔체30.7%。즉시접촉시험6충마풍수자유30 min내장미유전부살멸。즉시접촉감염시험마풍수자유원액감충솔체70.97%，첨가분갑산변지감충솔위58.87%，단일첨가월계담동감충솔체77.42%，첨가분갑산변지、린분이갑산이정지、월계담동감충솔체100%。결론95%을순회류、료액비1∶8、2 h제취공예위마풍수자유최가제취공예。마풍수자유유교호적혈흡충미유살멸작용。
Objective To evaluate the effect of Jatropha see soma japonicum so as to screen the optimum process and formulations. Methods The cercaria directly contacting tests with Jatro-pha seed oils extracted by 6 different extraction processes were carried out,and the mouse immediate contacting cercaria infection trials with different formulations of Jatropha seed oil and various additives were performed. Results With 95%ethanol,the ratio of material to liquid being 1∶8,and 2 h extraction,the oil extraction rate was 30.7%. The cercaria directly contacting tests showed that 6 kinds of Jatropha seed oil killed all cercaria within 30 min. In the mouse immediate contacting cercaria infection trials,the worm declined rate of Jatropha seed oil liquid was 70.97%,and the worm declined rate of the sample added with benzyl benzoate was 58.87%,and the worm declined rate of the sample added with laurocapram was 77.42%. The worm declined rate of the sam-ples added with benzyl benzoate,dibutyl phthalate and laurocapram was 100%. Conclusion The process with 95%ethanol,the ratio of material to liquid being 1∶8,and 2 h extraction is the optimum,and the Jatropha seed oil has a good killing schistosome cercaria effect.