CAJ | 학술논문

目的：观察Osterix在调控脊椎松质骨骨量的作用及机制。方法以Osterix敲除小鼠和Osterix转基因小鼠为观察对象，采用X线摄像、显微电子计算机X线断层摄影术（Micro-CT）和苏木素-伊红（HE）染色观察Osterix过表达或敲除后小鼠腰椎骨量的变化，抗酒石酸酸性磷酸酶（T RA P ）染色观察破骨细胞的变化。免疫组织化学检测核因子-κB受体活化因子配体（RANKL）表达情况的变化。结果 Osterix转基因小鼠腰椎骨量没有明显变化，而X线片、Micro-CT 和 HE染色检测显示Os-terix敲除小鼠在出生后12周脊椎密度和骨量增加；T RA P染色显示腰椎中破骨细胞数量减少；免疫组织化学染色检测显示其椎体中RANKL表达水平降低。结论 Osterix在调控脊椎松质骨骨量中起到重要作用。
목적：관찰Osterix재조공척추송질골골량적작용급궤제。방법이Osterix고제소서화Osterix전기인소서위관찰대상，채용X선섭상、현미전자계산궤X선단층섭영술（Micro-CT）화소목소-이홍（HE）염색관찰Osterix과표체혹고제후소서요추골량적변화，항주석산산성린산매（T RA P ）염색관찰파골세포적변화。면역조직화학검측핵인자-κB수체활화인자배체（RANKL）표체정황적변화。결과 Osterix전기인소서요추골량몰유명현변화，이X선편、Micro-CT 화 HE염색검측현시Os-terix고제소서재출생후12주척추밀도화골량증가；T RA P염색현시요추중파골세포수량감소；면역조직화학염색검측현시기추체중RANKL표체수평강저。결론 Osterix재조공척추송질골골량중기도중요작용。
Objective To observe the role of Osterix in controlling bone volume in vertebral body and to investigate the possible mechanism .Methods X-ray radiology ,micro CT and HE staining were used to evaluate the change of bone volume in both Osterix knockout and transgenic mice .TRAP staining was used to assess the activity of osteoclasts and immunohistochemistry was used to examine the expression level of RANKL .Results No obvious changes were found in Osterix transgenic mice ,while X-ray examina-tion ,micro CT and HE staining showed that the bone density and bone volume in the lumbar vertebral body increased significantly in OSX null mice 12 weeks after birth .TRAP staining showed that the number of osteoclasts decreased in OSX null mice .IHC re-vealed that the expression level of RANKL was down-regualted in OSX null mice .Conclusion Osterix play an important role in controlling bone volume of vertebral body in mice .