江苏师范大学学报(自然科学版)
江囌師範大學學報(自然科學版)
강소사범대학학보(자연과학판)
Journal of Xuzhou Normal University(Natural Science Edition)
2013年
4期
52-55
,共4页
邢根娣%胡秀彩%李雪%周洁%孟思妍%朱爱华%吕爱军
邢根娣%鬍秀綵%李雪%週潔%孟思妍%硃愛華%呂愛軍
형근제%호수채%리설%주길%맹사연%주애화%려애군
鲫鱼%温和气单胞菌%分离鉴定%药敏试验%16S rDNA
鯽魚%溫和氣單胞菌%分離鑒定%藥敏試驗%16S rDNA
즉어%온화기단포균%분리감정%약민시험%16S rDNA
crucian carp%A eromonas sobria%isolation and identification%antibiotic sensitivity%16S rDNA
从鲫鱼肠道分离纯化获得一株细菌,编号为XA-2,对其进行形态观察,并对理化特性、16S rDNA克隆测序及系统发育进化树构建等研究.结果表明,XA-2菌株为革兰氏阴性短杆菌,可发酵葡萄糖产气;进一步采用 PCR方法克隆16S rDNA序列,测得长度为1508 bp ;对系统发育进化树分析,发现XA-2菌株与温和气单胞菌(Aero-monas sobria)模式菌株NCIMB 12065的亲缘关系最近,同源性达99.73%,从而鉴定XA-2菌株为温和气单胞菌.采用27种抗生素进行药敏试验,结果显示该菌株对头孢噻吩、头孢噻肟、头孢克肟、头孢哌酮、新霉素、诺氟沙星、复方新诺明等药物敏感,对先锋霉素Ⅳ、阿莫西林、庆大霉素、卡那霉素、红霉素等药物不敏感.
從鯽魚腸道分離純化穫得一株細菌,編號為XA-2,對其進行形態觀察,併對理化特性、16S rDNA剋隆測序及繫統髮育進化樹構建等研究.結果錶明,XA-2菌株為革蘭氏陰性短桿菌,可髮酵葡萄糖產氣;進一步採用 PCR方法剋隆16S rDNA序列,測得長度為1508 bp ;對繫統髮育進化樹分析,髮現XA-2菌株與溫和氣單胞菌(Aero-monas sobria)模式菌株NCIMB 12065的親緣關繫最近,同源性達99.73%,從而鑒定XA-2菌株為溫和氣單胞菌.採用27種抗生素進行藥敏試驗,結果顯示該菌株對頭孢噻吩、頭孢噻肟、頭孢剋肟、頭孢哌酮、新黴素、諾氟沙星、複方新諾明等藥物敏感,對先鋒黴素Ⅳ、阿莫西林、慶大黴素、卡那黴素、紅黴素等藥物不敏感.
종즉어장도분리순화획득일주세균,편호위XA-2,대기진행형태관찰,병대이화특성、16S rDNA극륭측서급계통발육진화수구건등연구.결과표명,XA-2균주위혁란씨음성단간균,가발효포도당산기;진일보채용 PCR방법극륭16S rDNA서렬,측득장도위1508 bp ;대계통발육진화수분석,발현XA-2균주여온화기단포균(Aero-monas sobria)모식균주NCIMB 12065적친연관계최근,동원성체99.73%,종이감정XA-2균주위온화기단포균.채용27충항생소진행약민시험,결과현시해균주대두포새분、두포새우、두포극우、두포고동、신매소、낙불사성、복방신낙명등약물민감,대선봉매소Ⅳ、아막서림、경대매소、잡나매소、홍매소등약물불민감.
A bacterial strain was isolated from the intestine of crucian carp and named XA-2 .Its morphological , physiological and biochemical characteristics ,16S rDNA sequencing and analysis of phylogenetic tree were carried out in this study .The results showed that XA-2 strain was a short ,gram-negative bacillus and it can produce gas from glucose .Furthermore ,PCR method was applied to amplify 16S rDNA gene of the isolated strain ,and a 1508 bp DNA fragment of XA-2 strain was obtained;the phylogenetic tree analysis showed that XA-2 strain was most closely related to Aeromonas sobria(NCIMB 12065) ,the sequence homology was 99.73% ,taken together ,the strain XA-2 was identified as Aeromonas sobria .The antibiotic sensitivity analysis showed that XA-2 was sensitive to ceftofur ,cefotaxime ,cefixime ,cefoperazone ,neomycin ,norfloxacin ,trimethoprim ,and resistant to cephaloth-in ,amoxicillin ,gentamicin ,kanamycin ,erythromycin .