CAJ | 학술논문

环介导等温扩增是一种新的核酸扩增技术，具有简单、快速、特异性强的特点。针对多种食源性致病菌和病毒的环介导等温扩增检测方法已建立了起来，现在主要是发展成为发展中国家一些高发传染病的快速诊断方法，今后环介导等温扩增和微流体等技术的联用有望推动基因床边诊断的实现。本文针对环介导等温扩增技术过去、现在的应用和未来的发展方向展开了论述。
배개도등온확증시일충신적핵산확증기술，구유간단、쾌속、특이성강적특점。침대다충식원성치병균화병독적배개도등온확증검측방법이건립료기래，현재주요시발전성위발전중국가일사고발전염병적쾌속진단방법，금후배개도등온확증화미류체등기술적련용유망추동기인상변진단적실현。본문침대배개도등온확증기술과거、현재적응용화미래적발전방향전개료논술。
Loop-mediated isothermal amplification (LAMP) is an established nucleic acid amplification method offering a rapid, accurate, and cost-effective diagnosis of infectious diseases. This technology has been developed for a variety of pathogens, including bacteria and viruses. The current focus on LAMP methodology is to explore LAMP as a diagnostic system in resource-limited laboratories in developing countries, where many fatal tropical diseases are endemic. The combination of LAMP and novel microfluidic technologies may facilitate the realization of genetic point-of-care testing systems to be used in the near future. This review described the historical, current, and future developments of such technologies.