检验检疫学刊
檢驗檢疫學刊
검험검역학간
INSPECTION AND QUARANTINE SCIENCE
2013年
6期
32-38
,共7页
马小宁%田燕%宋小莉%张凤霞%贾迪%李淑娟%仲维科%刘汉霞
馬小寧%田燕%宋小莉%張鳳霞%賈迪%李淑娟%仲維科%劉漢霞
마소저%전연%송소리%장봉하%가적%리숙연%중유과%류한하
婴幼儿配方乳粉%1,3-二油酸2-棕榈酸甘油三酯%气相色谱(氢火焰检测器)%多模式进样口%定量检测
嬰幼兒配方乳粉%1,3-二油痠2-棕櫚痠甘油三酯%氣相色譜(氫火燄檢測器)%多模式進樣口%定量檢測
영유인배방유분%1,3-이유산2-종려산감유삼지%기상색보(경화염검측기)%다모식진양구%정량검측
Infant Formula%1,3-Dioleoyl 2-Palmitoyl Triglyceride%Gas Chromatography(FID)%Multimodal Injector%Quantification Determination
采用乙醚和石油醚提取出配方乳粉中的油脂，以C51：0为内标，通过带有多模式进样口进样器及氢火焰检测器的气相色谱仪对其中的1,3-二油酸2-棕榈酸甘油三酯进行定量分析。结果表明，在本方法的色谱条件下，配方乳粉的1,3-二油酸2-棕榈酸甘油三酯能与其他C52系列脂肪酸组分很好的分离，方法重现性良好， RSD为2.93%，回收率为92.4±4.5%。该方法准确可靠，可准确测定配方乳粉中OPO的含量。
採用乙醚和石油醚提取齣配方乳粉中的油脂，以C51：0為內標，通過帶有多模式進樣口進樣器及氫火燄檢測器的氣相色譜儀對其中的1,3-二油痠2-棕櫚痠甘油三酯進行定量分析。結果錶明，在本方法的色譜條件下，配方乳粉的1,3-二油痠2-棕櫚痠甘油三酯能與其他C52繫列脂肪痠組分很好的分離，方法重現性良好， RSD為2.93%，迴收率為92.4±4.5%。該方法準確可靠，可準確測定配方乳粉中OPO的含量。
채용을미화석유미제취출배방유분중적유지，이C51：0위내표，통과대유다모식진양구진양기급경화염검측기적기상색보의대기중적1,3-이유산2-종려산감유삼지진행정량분석。결과표명，재본방법적색보조건하，배방유분적1,3-이유산2-종려산감유삼지능여기타C52계렬지방산조분흔호적분리，방법중현성량호， RSD위2.93%，회수솔위92.4±4.5%。해방법준학가고，가준학측정배방유분중OPO적함량。
Establish quantification determination method for 1,3-Dioleoyl 2-palmitoyl triglyceride (OPO) in infant formula in order to meet the requirement of the product and to supply technical support for guarantee the infant formula safety. OPO in infant formula were extracted by ethyl ether and petroleum ether, and then OPO was determined by gas chromatography (GC) equipped with multimodal injector (MMI) sample injector and FID detector with C51:0 as internal standard. The results showed that OPO was separated from other C52 compounds very well under the GC condition. The reproducibility of the method is good, the relative standard deviation was 2.93%and the recovery was 92.4±4.5%. The method was accurate and stable to determine the concentration of OPO in the infant formula.
