CAJ | 학술논문

目的：建立白鹤灵芝药材中伞形花内酯质量分数测定的方法．方法：色谱柱XtimateTM -C18（250 mm ×4．6 mm ×5μm）；流动相：V（0．1％磷酸水溶液）∶V（乙腈）＝75∶25；流速为0．8 mL/min；检测波长318 nm；柱温25℃．结果：伞形花内酯质量分数在一定范围内线性关系良好（r＝0．9997），加标回收率为100．12％，RSD＝2．16％（n＝6），不同批次白鹤灵芝中伞形花内酯质量分数差异明显．结论：建立的方法操作简单，精密度好，结果准确，可用于白鹤灵芝中伞形花内酯质量分数的测定．
목적：건립백학령지약재중산형화내지질량분수측정적방법．방법：색보주XtimateTM -C18（250 mm ×4．6 mm ×5μm）；류동상：V（0．1％린산수용액）∶V（을정）＝75∶25；류속위0．8 mL/min；검측파장318 nm；주온25℃．결과：산형화내지질량분수재일정범위내선성관계량호（r＝0．9997），가표회수솔위100．12％，RSD＝2．16％（n＝6），불동비차백학령지중산형화내지질량분수차이명현．결론：건립적방법조작간단，정밀도호，결과준학，가용우백학령지중산형화내지질량분수적측정．
Objective:To develop a HPLC method for determinating the umbelliferone in Rhinacanthus nasu-tus (L.) Lindau.Methods:XtimateTM -C18 (250 mm ×4.6 mm ×5 μm); mobile phase:0.1% aqueous phos-phoric acid /acetonitrile (75∶25);flow rate 0.8 mL/min;detection wavelength:318 nm;the column temperature was controlled at 25℃.Results:The umbelliferone had a good linear relationship in a certain range (r=0.999 7), recovery rates were in the range of 100.12%, RSD=2.16%(n=6), the content of umbelliferone varied in differ-ent collection periods .Conclusions:The proposed method was found to be simple , precise and accurate and suit-able for the simultaneous determination of umbelliferone in Rhinacanthus nasutus ( L.) Lindau.