中国现代药物应用
中國現代藥物應用
중국현대약물응용
CHINESE JOURNAL OF MODERN DRUG APPLICATION
2014年
7期
1-2,3
,共3页
虎杖提取物%CCI%神经病理性疼痛%小胶质细胞
虎杖提取物%CCI%神經病理性疼痛%小膠質細胞
호장제취물%CCI%신경병이성동통%소효질세포
Polygonum cuspidatum%CCI%Neuropathic pain%Microglial cell
目的：研究虎杖提取物对神经病理性疼痛模型大鼠的镇痛作用,并探讨其作用机制。方法雄性SD大鼠,随机分为对照组和坐骨神经缩狭模型(chronic construction injury, CCI)组。CCI术后14 d,应用Von Frey法测定机械刺激缩足反射阈值(mechanical withdrawal threshold, MWT),观察口服不同剂量的虎杖提取物对CCI大鼠机械性痛觉超敏的影响。并考察不同剂量虎杖提取物对CCI大鼠脊髓p-ERK、p-P38水平及小胶质细胞活化的影响。结果虎杖提取物可明显升高CCI大鼠术侧的MWT值(P<0.05),而对正常大鼠的MWT值没有显著影响(P>0.05)。同时虎杖提取物可显著抑制CCI模型大鼠脊髓ERK和p38的磷酸化,并显著抑制IBA-1表达增加。结论虎杖提取物对CCI诱导的机械性痛觉超敏具有显著性的抑制作用,且镇痛作用维持时间长于吗啡,其机制可能是通过抑制脊髓水平ERK和p38的磷酸化,及通过抑制小胶质细胞活化而实现。
目的：研究虎杖提取物對神經病理性疼痛模型大鼠的鎮痛作用,併探討其作用機製。方法雄性SD大鼠,隨機分為對照組和坐骨神經縮狹模型(chronic construction injury, CCI)組。CCI術後14 d,應用Von Frey法測定機械刺激縮足反射閾值(mechanical withdrawal threshold, MWT),觀察口服不同劑量的虎杖提取物對CCI大鼠機械性痛覺超敏的影響。併攷察不同劑量虎杖提取物對CCI大鼠脊髓p-ERK、p-P38水平及小膠質細胞活化的影響。結果虎杖提取物可明顯升高CCI大鼠術側的MWT值(P<0.05),而對正常大鼠的MWT值沒有顯著影響(P>0.05)。同時虎杖提取物可顯著抑製CCI模型大鼠脊髓ERK和p38的燐痠化,併顯著抑製IBA-1錶達增加。結論虎杖提取物對CCI誘導的機械性痛覺超敏具有顯著性的抑製作用,且鎮痛作用維持時間長于嗎啡,其機製可能是通過抑製脊髓水平ERK和p38的燐痠化,及通過抑製小膠質細胞活化而實現。
목적：연구호장제취물대신경병이성동통모형대서적진통작용,병탐토기작용궤제。방법웅성SD대서,수궤분위대조조화좌골신경축협모형(chronic construction injury, CCI)조。CCI술후14 d,응용Von Frey법측정궤계자격축족반사역치(mechanical withdrawal threshold, MWT),관찰구복불동제량적호장제취물대CCI대서궤계성통각초민적영향。병고찰불동제량호장제취물대CCI대서척수p-ERK、p-P38수평급소효질세포활화적영향。결과호장제취물가명현승고CCI대서술측적MWT치(P<0.05),이대정상대서적MWT치몰유현저영향(P>0.05)。동시호장제취물가현저억제CCI모형대서척수ERK화p38적린산화,병현저억제IBA-1표체증가。결론호장제취물대CCI유도적궤계성통각초민구유현저성적억제작용,차진통작용유지시간장우마배,기궤제가능시통과억제척수수평ERK화p38적린산화,급통과억제소효질세포활화이실현。
Objective To study the analgesic effect and mechanism of polygonum cuspidatum extract in the neuropathic pain using rat chronic constriction (CCI) mode. Methods The SD rats were randomly divided into control and model groups. After 14 days of CCI, we observe the effect of different doses of polygonum cuspidatum extract on rat mechanical allodynia using von Frey flinching reflex threshold (mechanical withdrawal threshold, MWT), The levels of p-ERK, p-P38 and the activation of microglial cells in rat spinals were measured by Western blotting. Results polygonum cuspidatum extract can significantly increase the value of MWT in CCI rats (P<0.05), whereas had no significant effect in normal rats (P>0.05). Meanwhile, polygonum cuspidatum extract significantly inhibited the phosphorylation of ERK and p38, and inhibited the expression of IBA-1 in CCI rat. Conclusion Polygonum cuspidatum extract can inhibit the mechanical allodynia in CCI rat, and the duration is longer than the analgesic effect of morphine. The mechanism may be associated with the inhibition of the phosphorylation of ERK and p38, and inhibition of microglial cell activation in rat spinal.
