CAJ | 학술논문

目的：检测慢性阻塞性肺疾病急性加重期（AE-COPD）患者呼吸道内具核梭杆菌（Fn.）的定植，探讨Fn.与AE-COPD间的关系。方法选择2008年10月至2009年4月中国医科大学附属第一医院、盛京医院确诊为AE-COPD的患者53例，采集呼吸道分泌物，提取细菌DNA，应用SYBR@GreenⅠPremix Ex TaqTM模式的实时PCR技术，对Fn.进行定量检测，并计算其所占总菌的比例。同时记录患者FEV1占预计值比例（%）及口腔牙周指标。结果53例样本中有32例检出Fn.，Fn.检出率为60.38%，占总菌的比例为（44.58±16.47）%。Fn.相对含量与FEV1占预计值比例呈负相关关系（P<0.05）。Fn.相对含量与简化口腔卫生指数及附着丧失水平呈正相关关系（P<0.05）；与牙龈出血指数、探诊深度、缺失牙数无相关性（P>0.05）。结论 AE-COPD患者呼吸道内存在Fn.， Fn.相对含量随着肺功能的减弱而增加；Fn.与AE-COPD密切相关。
목적：검측만성조새성폐질병급성가중기（AE-COPD）환자호흡도내구핵사간균（Fn.）적정식，탐토Fn.여AE-COPD간적관계。방법선택2008년10월지2009년4월중국의과대학부속제일의원、성경의원학진위AE-COPD적환자53례，채집호흡도분비물，제취세균DNA，응용SYBR@GreenⅠPremix Ex TaqTM모식적실시PCR기술，대Fn.진행정량검측，병계산기소점총균적비례。동시기록환자FEV1점예계치비례（%）급구강아주지표。결과53례양본중유32례검출Fn.，Fn.검출솔위60.38%，점총균적비례위（44.58±16.47）%。Fn.상대함량여FEV1점예계치비례정부상관관계（P<0.05）。Fn.상대함량여간화구강위생지수급부착상실수평정정상관관계（P<0.05）；여아간출혈지수、탐진심도、결실아수무상관성（P>0.05）。결론 AE-COPD환자호흡도내존재Fn.， Fn.상대함량수착폐공능적감약이증가；Fn.여AE-COPD밀절상관。
Objective To detect the presence of Fusobacterium nucleatum(Fn.)in the acute exacerbation of chronic ob-structive pulmonary disease(AE-COPD)and to investigate its relationship with FEV1 and periodontal index. Methods The sputum samples of AE-COPD were collected and were extracted DNA of bacterium. The amount of Fn. and the to-tal number of bacterial cells were determined by real-time PCR based on SYBR@Green I Premix Ex TaqTM. FEV1 and periodontal parameters of each AE-COPD were recorded,and then investigate the relationship between Fn. and FEV1 and periodontal index. The data were statistically analyzed using SPSS17.0 software. Results The detection rate of Fn. was 60.38%and the relative quantity of Fn. was(44.58 ± 16.47)%. There was negative correlation between the relative quantity of Fn. and FEV1(P<0.05). There was positive correlation between the relative quantity of Fn. and simplified oral hygiene index and clinical attachment loss(P<0.05). Conclusion Fn. can colonize in the sputum samples in the AE-COPD and colonization of Fn. is relevant to FEV1 and periodontal parameters.