CAJ | 학술논문

[目的]探讨脯氨酸羟化酶抑制剂---二甲基乙二酰基甘氨酸（dimethyloxaloylglycine，DMOG）对小鼠间充质干细胞（mouse mesenchymal stem cel s，mMSCs）的动员作用。[方法]ICR小鼠随机分为对照组及3个DMOG动员剂量组（20、40、80mg·kg-1）。不同剂量的DMOG每天一次在固定的时间段腹腔内注射入ICR小鼠，对照组给予相同体积的生理盐水。采用流式细胞术与成纤维细胞集落形成单位法检测不同浓度DMOG处理后小鼠外周血与骨髓中MSCs数量，Western blotting法检测骨髓细胞中HIF-1α、SDF-1α及VEGF蛋白的表达，ELISA法检测骨髓上清及外周血清中SDF-1α及VEGF蛋白的浓度。[结果]流式细胞术与成纤维细胞集落形成单位法检测发现不同剂量的DMOG处理7d后小鼠外周血与骨髓中MSCs数量较对照组显著增加（P＜0.05），且CD45-CD90+细胞群比例升高（P＜0.05）。同时Western blotting法及ELISA法检测发现小鼠骨髓HIF-1α、SDF-1α表达上调，VEGF浓度升高（P＜0.05）。[结论]DMOG对小鼠间充质干细胞具有动员作用，可能与通过上调HIF-1α，从而调控其下游相关通路有关。
[목적]탐토포안산간화매억제제---이갑기을이선기감안산（dimethyloxaloylglycine，DMOG）대소서간충질간세포（mouse mesenchymal stem cel s，mMSCs）적동원작용。[방법]ICR소서수궤분위대조조급3개DMOG동원제량조（20、40、80mg·kg-1）。불동제량적DMOG매천일차재고정적시간단복강내주사입ICR소서，대조조급여상동체적적생리염수。채용류식세포술여성섬유세포집락형성단위법검측불동농도DMOG처리후소서외주혈여골수중MSCs수량，Western blotting법검측골수세포중HIF-1α、SDF-1α급VEGF단백적표체，ELISA법검측골수상청급외주혈청중SDF-1α급VEGF단백적농도。[결과]류식세포술여성섬유세포집락형성단위법검측발현불동제량적DMOG처리7d후소서외주혈여골수중MSCs수량교대조조현저증가（P＜0.05），차CD45-CD90+세포군비례승고（P＜0.05）。동시Western blotting법급ELISA법검측발현소서골수HIF-1α、SDF-1α표체상조，VEGF농도승고（P＜0.05）。[결론]DMOG대소서간충질간세포구유동원작용，가능여통과상조HIF-1α，종이조공기하유상관통로유관。
[Objective]To explore the effect of prolylhydroxylase inhibitor-dimethyloxal ylglycine(DMOG) on mesenchymal stem cells mobilization in mice. [Methods]ICR mice were randomly assigned into 3 mobilization groups(20, 40, 80mg·kg-1 DMOG) and a control group. ICR mice were treated with differ-ent doses of DMOG daily in a fixed time interval by intraperitoneal injection, while the control group was given the same volume of physiological saline in-stead. We used flow cytometry and CFU-F assay to determine the number of MSCs in peripheral blood and bone marrow in each group. We also detected the expression of HIF-1α, SDF-1αand VEGF both in peripheral blood and bone marrow in each group by Western blotting and ELISA.[Results]The de-tection of flow cytometry and CFU-F assay showed that the number of MSCs in peripheral blood and bone marrow increased significantly in mice treated with different doses of DMOG for 7 days, with higher percentages of CD45-CD90+cells. In addition, Western blotting and ELISA analysis identified that the protein level of HIF-1αand SDF-1αand the concentrations of VEGF were increased in bone marrow. [Conclusion] DMOG can induce MSC mobilization, possibly via up-regulating the expression of HIF-1αand activating its downstream pathways.