大众科技
大衆科技
대음과기
DAZHONG KEJI
2013年
12期
124-127
,共4页
甲状腺切除术%嗅球切除术%难治性抑郁症%甲状腺功能低下%实验动物模型
甲狀腺切除術%嗅毬切除術%難治性抑鬱癥%甲狀腺功能低下%實驗動物模型
갑상선절제술%후구절제술%난치성억욱증%갑상선공능저하%실험동물모형
Thyroidectomy%olfactory bulbectomy%treatment-resistant depression%hypothyroidism%experimental animal model
目的：探讨建立伴有甲状腺功能低下的难治性抑郁症实验动物模型的可行性。方法：将30只雄性SD大鼠随机分成3组，每组10只：模型组、假手术组、正常对照组。模型组大鼠首先接受甲状腺切除术，7天后再次接受双侧嗅球切除术；对假手术组大鼠除了不切除甲状腺与双侧嗅球以外，其余操作均与模型组的完全相同；对正常对照组大鼠则不进行任何处理。在最终手术后的第2周，采用旷场试验、强迫游泳试验与Morris水迷宫测试各组大鼠的行为变化。结果：与正常对照组或假手术组大鼠相比较，模型组大鼠长生相对停滞、体重明显减轻，并出现焦虑样和抑郁样行为，空间学习记忆能力明显下降。模型组大鼠的这些指标与正常对照组或假手术组大鼠的差异均有统计学意义（P＜0.01），但正常对照组与假手术组之间的差异则无统计学意义（P＞0.05）。结论：本研究全程应用两种外科手术建立的大鼠模型在原因与行为变化方面，可较好地模拟出伴有甲状腺功能低下的难治性抑郁症的临床特征，故其可能有潜力成为这类难治性抑郁症激越亚型的一种实验动物模型，也为后续开展联合药物治疗与揭示相关药理学机制的研究打下了一个平台。
目的：探討建立伴有甲狀腺功能低下的難治性抑鬱癥實驗動物模型的可行性。方法：將30隻雄性SD大鼠隨機分成3組，每組10隻：模型組、假手術組、正常對照組。模型組大鼠首先接受甲狀腺切除術，7天後再次接受雙側嗅毬切除術；對假手術組大鼠除瞭不切除甲狀腺與雙側嗅毬以外，其餘操作均與模型組的完全相同；對正常對照組大鼠則不進行任何處理。在最終手術後的第2週，採用曠場試驗、彊迫遊泳試驗與Morris水迷宮測試各組大鼠的行為變化。結果：與正常對照組或假手術組大鼠相比較，模型組大鼠長生相對停滯、體重明顯減輕，併齣現焦慮樣和抑鬱樣行為，空間學習記憶能力明顯下降。模型組大鼠的這些指標與正常對照組或假手術組大鼠的差異均有統計學意義（P＜0.01），但正常對照組與假手術組之間的差異則無統計學意義（P＞0.05）。結論：本研究全程應用兩種外科手術建立的大鼠模型在原因與行為變化方麵，可較好地模擬齣伴有甲狀腺功能低下的難治性抑鬱癥的臨床特徵，故其可能有潛力成為這類難治性抑鬱癥激越亞型的一種實驗動物模型，也為後續開展聯閤藥物治療與揭示相關藥理學機製的研究打下瞭一箇平檯。
목적：탐토건립반유갑상선공능저하적난치성억욱증실험동물모형적가행성。방법：장30지웅성SD대서수궤분성3조，매조10지：모형조、가수술조、정상대조조。모형조대서수선접수갑상선절제술，7천후재차접수쌍측후구절제술；대가수술조대서제료불절제갑상선여쌍측후구이외，기여조작균여모형조적완전상동；대정상대조조대서칙불진행임하처리。재최종수술후적제2주，채용광장시험、강박유영시험여Morris수미궁측시각조대서적행위변화。결과：여정상대조조혹가수술조대서상비교，모형조대서장생상대정체、체중명현감경，병출현초필양화억욱양행위，공간학습기억능력명현하강。모형조대서적저사지표여정상대조조혹가수술조대서적차이균유통계학의의（P＜0.01），단정상대조조여가수술조지간적차이칙무통계학의의（P＞0.05）。결론：본연구전정응용량충외과수술건립적대서모형재원인여행위변화방면，가교호지모의출반유갑상선공능저하적난치성억욱증적림상특정，고기가능유잠력성위저류난치성억욱증격월아형적일충실험동물모형，야위후속개전연합약물치료여게시상관약이학궤제적연구타하료일개평태。
Objective: To examine whether surgical paradigm (TX+OBX) of thyroidectomy (TX) and olfactory bulbectomy (OBX) performed on rats potentially becomes an animal model of treatment-resistant depression with hypothyroidism. Methods: Thirty Sprague-Dawley adult male rats were randomly divided into three groups with ten rats in each group:model, sham and intact. Both TX and OBX were subsequently performed on rats in model group. After two weeks of OBX operation, open field test, forced swimming test and Morris water maze were used to measure the behavioral changes shown by rats in the three groups. Results: Rats in model group had a lower body weight with a retardation of body growth, and displayed an anxiety-like or agitated depression-like behavior as well as poor ability of spatial learning and memory. These parameter changes in model group rats were statistically different from those in intact group rats or in sham group rats(P<0.01), but no statistical differences of the above parameters existed between intact group rats and sham group rats(P>0.05). Conclusion based on the above data, the pattern of TX+OBX rats largely mimics the etiology and the symptoms of treatment-resistant depression with hypothyroidism , and therefore it potentially becomes a possible animal model of agitated subtype in treatment-resistant depression with hypothyroidism, and may be used as a platform for subsequent intervention of combined thyroid hormone and antidepressant drugs and for exploring relative pharmacological mechanisms of antidepressant therapy.
