CAJ | 학술논문

目的：观察经PhaP-RGD融合蛋白修饰的3-羟基丁酸和3-羟基己酸的共聚酯（PHBHHx）生物材料与人鼻中隔软骨细胞的生物相容性。方法体外培养人鼻中隔软骨细胞，接种于经PhaP-RGD融合蛋白修饰的PHBHHx膜性材料上进行体外培养，通过MTT比色法、DAPI染色、扫描电镜、甲苯胺蓝染色等实验方法观察在体外条件下生物修饰后的PHBHHx膜与鼻中隔软骨细胞的生物相容性。结果经PhaP-RGD融合蛋白修饰的PHBHHx膜表面接触角变小，亲水性增加；人鼻中隔软骨细胞在蛋白修饰后的生物膜材料表面能保持较高的增殖率；经蛋白修饰的PHBHHx膜表面的软骨细胞活力明显高于细胞培养板及未经蛋白修饰的PHBHHx膜上的软骨细胞；甲苯氨蓝染色发现经PhaP-RGD修饰的PHBHHx膜能促进软骨细胞分泌胞外基质糖胺多糖。结论经PhaP-RGD融合蛋白修饰的PHBHHx与软骨细胞的生物相容性良好，是一种较理想的软骨组织工程支架材料。
목적：관찰경PhaP-RGD융합단백수식적3-간기정산화3-간기기산적공취지（PHBHHx）생물재료여인비중격연골세포적생물상용성。방법체외배양인비중격연골세포，접충우경PhaP-RGD융합단백수식적PHBHHx막성재료상진행체외배양，통과MTT비색법、DAPI염색、소묘전경、갑분알람염색등실험방법관찰재체외조건하생물수식후적PHBHHx막여비중격연골세포적생물상용성。결과경PhaP-RGD융합단백수식적PHBHHx막표면접촉각변소，친수성증가；인비중격연골세포재단백수식후적생물막재료표면능보지교고적증식솔；경단백수식적PHBHHx막표면적연골세포활력명현고우세포배양판급미경단백수식적PHBHHx막상적연골세포；갑분안람염색발현경PhaP-RGD수식적PHBHHx막능촉진연골세포분비포외기질당알다당。결론경PhaP-RGD융합단백수식적PHBHHx여연골세포적생물상용성량호，시일충교이상적연골조직공정지가재료。
Objective To explore the biocompatibility of PHBHHx biomaterial coated with PhaP-RGD fusion protein on human nasal septal chondrocytes. Methods The human nasal septal chondrocytes were cultured and seeded onto the PHBHHx biomaterials modified with PhaP-RGD fusion protein in vitro. The biocompatibility of modified PHBHHx on human nasal septal chondrocytes was detected by MTT assay, DAPI staining, scanning electron microscopy and toluidine blue staining. Results The contact angle of modified PHBHHx was smaller and the surface hydrophilicity increased; The human nasal septal chondrocytes on modified maintained a high proliferation rate. The viability of chondrocytes on modified PHBHHx film was significantly higher than that on PHBHHx film and cell culture plate. It is discovered that PHBHHx film coated with PhaP-RGD can promote the secretion of extracellular matrix of cartilage--glycosaminoglycan by toluidine blue staining. Conclusion PHBHHx coated with PhaP-RGD fusion protein has better biocompatibility on chondrocytes, and is an ideal scaffold material for cartilage tissue engineering.