CAJ | 학술논문

目的探讨大肠癌中microRNA-93的表达及其与临床病理因素的关系。方法取42例大肠癌及癌旁正常黏膜组织标本，采用茎环逆转录实时荧光定量PCR方法检测大肠癌及癌旁正常黏膜组织中microRNA-93的表达水平，并比较分析其在不同临床病理因素间的表达差异。结果（1）microRNA-93在大肠癌组织中的表达水平显著高于癌旁正常黏膜组织，差异有统计学意义（P＜0.01）；（2）microRNA-93的表达与大肠癌淋巴结转移和浸润深度有关（P＜0.05或0.01）；而与患者的年龄、性别、肿瘤大小、远处转移、分化程度无关（均P＞0.05）；（3）microRNA-93评价大肠癌淋巴结转移的敏感性和特异性分别为0.867和0.556，评价大肠癌浸润深度的敏感性和特异性为0.800和0.833。结论 microRNA-93表达上调可能与大肠癌的发生、发展有关，microRNA-93可作为评价大肠癌淋巴结转移和浸润深度的辅助指标。
목적탐토대장암중microRNA-93적표체급기여림상병리인소적관계。방법취42례대장암급암방정상점막조직표본，채용경배역전록실시형광정량PCR방법검측대장암급암방정상점막조직중microRNA-93적표체수평，병비교분석기재불동림상병리인소간적표체차이。결과（1）microRNA-93재대장암조직중적표체수평현저고우암방정상점막조직，차이유통계학의의（P＜0.01）；（2）microRNA-93적표체여대장암림파결전이화침윤심도유관（P＜0.05혹0.01）；이여환자적년령、성별、종류대소、원처전이、분화정도무관（균P＞0.05）；（3）microRNA-93평개대장암림파결전이적민감성화특이성분별위0.867화0.556，평개대장암침윤심도적민감성화특이성위0.800화0.833。결론 microRNA-93표체상조가능여대장암적발생、발전유관，microRNA-93가작위평개대장암림파결전이화침윤심도적보조지표。
Objective To investigate the expression of microRNA- 93 in colorectal carcinoma and its relation to clinico-pathological features of the cancer. Methods The expression of microRNA- 93 stem- loop real- time RT- PCR in cancer tissue and matched pericancerous tissue specimens from 42 patients with colorectal carcinoma. The relationship between microR-NA- 93 expression and clinicopathologic features of colorectal carcinoma was analyzed. Results The expression level of mi-croRNA- 93 was significantly higher in colorectal carcinoma than that in pericancerous tissues (P<0.05). The expression of mi-croRNA- 93 was positively correlated with the lymph node metastasis and depth of tumor invasion (P<0.05 or 0.01), not with age, sex, tumor size, distant metastasis and celldifferentiation(P>0.05). The sensitivity and specificity of microRNA- 93 expression for evaluation of lymph node metastasis were 0.867 and 0.556 respectively;those for evaluation of tumor invasion depth were 0.800 and 0.833, respectively. Conclusion The over- expression of microRNA- 93 may be associated with development and progres-sion of colorectal carcinoma. The expression of microRNA- 93 might be used as a adjuvant parameter for predicting the lymph node metastasis and depth of tumor invasion in colorectal carcinoma.