天然产物研究与开发
天然產物研究與開髮
천연산물연구여개발
NATURAL PRODUCT RESEARCH AND DEVELOPMENT
2013年
1期
1-6,59
,共7页
孙玉林%陈茵%徐新亚%张晓勇%郑智慧%农旭华%鲍洁%漆淑华
孫玉林%陳茵%徐新亞%張曉勇%鄭智慧%農旭華%鮑潔%漆淑華
손옥림%진인%서신아%장효용%정지혜%농욱화%포길%칠숙화
海洋真菌%Penicillium oxalicum SCSGAF 0023%次生代谢产物%抗污损%酶抑制活性
海洋真菌%Penicillium oxalicum SCSGAF 0023%次生代謝產物%抗汙損%酶抑製活性
해양진균%Penicillium oxalicum SCSGAF 0023%차생대사산물%항오손%매억제활성
利用pTLC、硅胶、Sephadex LH-20及半制备HPLC等柱色谱手段,从海洋真菌Penicillium oxalicum SCSGAF 0023的次生代谢产物中分离得到16个化合物,波谱学数据分析鉴定为n-butyl isobutyl terephthalate(1),dibutyl terephthalate(2),1,3,7-trihydroxy-6-methylanthraquinone(3),1,6,7-trihydroxy-3-methoxy-anthraquinone (4),isorhodoptilometrin (5),citreorosein (6),emodin (7),methyl-3,8-dihydroxy-6-methyl-9-oxo-9H-xanthene-1-carboxylate (8),pinselin (9),secalonic acid D(10),quinolactacin C1(11),quinolactacin C2(12),quinolactacin A1 (13),quinolactacin A2 (14),quinolactacin B1(15),3-methyl-IH-indole-2-carboxylic acid (16).其中,化合物1为新天然产物,化合物5和7表现出强的抗草苔虫幼虫附着活性,EC50值分别为3.8和6.0 μ.g/mL;化合物10和15分别对PTPIB和cathepsin B显示中等的酶抑制活性,IC50值分别为24.0和16.0 μM.
利用pTLC、硅膠、Sephadex LH-20及半製備HPLC等柱色譜手段,從海洋真菌Penicillium oxalicum SCSGAF 0023的次生代謝產物中分離得到16箇化閤物,波譜學數據分析鑒定為n-butyl isobutyl terephthalate(1),dibutyl terephthalate(2),1,3,7-trihydroxy-6-methylanthraquinone(3),1,6,7-trihydroxy-3-methoxy-anthraquinone (4),isorhodoptilometrin (5),citreorosein (6),emodin (7),methyl-3,8-dihydroxy-6-methyl-9-oxo-9H-xanthene-1-carboxylate (8),pinselin (9),secalonic acid D(10),quinolactacin C1(11),quinolactacin C2(12),quinolactacin A1 (13),quinolactacin A2 (14),quinolactacin B1(15),3-methyl-IH-indole-2-carboxylic acid (16).其中,化閤物1為新天然產物,化閤物5和7錶現齣彊的抗草苔蟲幼蟲附著活性,EC50值分彆為3.8和6.0 μ.g/mL;化閤物10和15分彆對PTPIB和cathepsin B顯示中等的酶抑製活性,IC50值分彆為24.0和16.0 μM.
이용pTLC、규효、Sephadex LH-20급반제비HPLC등주색보수단,종해양진균Penicillium oxalicum SCSGAF 0023적차생대사산물중분리득도16개화합물,파보학수거분석감정위n-butyl isobutyl terephthalate(1),dibutyl terephthalate(2),1,3,7-trihydroxy-6-methylanthraquinone(3),1,6,7-trihydroxy-3-methoxy-anthraquinone (4),isorhodoptilometrin (5),citreorosein (6),emodin (7),methyl-3,8-dihydroxy-6-methyl-9-oxo-9H-xanthene-1-carboxylate (8),pinselin (9),secalonic acid D(10),quinolactacin C1(11),quinolactacin C2(12),quinolactacin A1 (13),quinolactacin A2 (14),quinolactacin B1(15),3-methyl-IH-indole-2-carboxylic acid (16).기중,화합물1위신천연산물,화합물5화7표현출강적항초태충유충부착활성,EC50치분별위3.8화6.0 μ.g/mL;화합물10화15분별대PTPIB화cathepsin B현시중등적매억제활성,IC50치분별위24.0화16.0 μM.
